Neolitsea sericea essential oil attenuates LPS-induced inflammation in RAW 264.7 macrophages by suppressing NF-kappaB and MAPK activation.

Abstract

The chemical composition and anti-inflammatory activities of hydrodistilled essential oil from Neolitsea sericea leaves (NSE) have been investigated for the first time. The chemical constituents of NSE were analysed by GC-MS and found to include sericenine (32.3%), sabinene (21.0%), trans-beta-ocimene (13.3%), beta-caryophyllene (4.8%), and 4-terpineol (4.2%). The effects of NSE on nitric oxide (NO), prostaglandin E2 (PGE2), tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6 production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages were also examined. Pro-inflammatory cytokine and mediator tests indicated that NSE has excellent dose-dependent inhibitory activities. To further examine the mechanism responsible for the inhibition of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression by NSE, we examined the effect of NSE on nuclear factor-kappaB (NF-kappaB) activation and the phosphorylation of mitogen-activated protein kinases (MAPK). NSE inhibited NF-kappaB activation by LPS, and this was associated with the abrogation of IkappaB-alpha phosphorylation and subsequent decreases in nuclear p50 and p65 protein levels. Further, the phosphorylation of p38, ERK and JNK was suppressed by NSE in a concentration-dependent manner. These results suggest that NSE exerts anti-inflammatory effects in LPS-stimulated RAW 264.7 macrophages by inhibition of NF-kappaB activation and MAPK phosphorylation, and, therefore, may be useful for treatment of inflammatory diseases.

• Authors: Weon-Jong Yoon, Ji-Young Moon, Ji-Yong Kang, Gi-Ok Kim, Nam Ho Lee, Chang-Gu Hyun
• Journal: Natural product communications (Nat Prod Commun) Vol. 5 Issue 8 Pg. 1311-6 (Aug 2010) ISSN: 1934-578X [Print] United States
• PMID: 20839643 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Interleukin-6
• Lipopolysaccharides
• NF-kappa B
• Oils, Volatile
• Tumor Necrosis Factor-alpha
• Nitric Oxide
• Mitogen-Activated Protein Kinases
• Dinoprostone

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cell Line
• Dinoprostone (biosynthesis)
• Interleukin-6 (biosynthesis)
• Lauraceae (chemistry)
• Lipopolysaccharides (pharmacology)
• Macrophages (drug effects)
• Mice
• Mitogen-Activated Protein Kinases (antagonists & inhibitors)
• NF-kappa B (antagonists & inhibitors)
• Nitric Oxide (biosynthesis)
• Oils, Volatile (pharmacology)
• Tumor Necrosis Factor-alpha (biosynthesis)