Walnut peptide WEKPPVSH in alleviating oxidative stress and inflammation in lipopolysaccharide-activated BV-2 microglia via the Nrf2/HO-1 and NF-κB/p38 MAPK pathways.

Abstract

The peptide WEKPPVSH from walnut protein hydrolyzate was used to evaluate the antioxidant and anti-inflammatory protective effect on lipopolysaccharide (LPS)-activated BV-2 microglia and its possible mechanism. The results indicated that WEKPPVSH significantly decreased nitric oxide (NO) and reactive oxygen species (ROS) generation in a dose-dependent manner, and significantly up-regulated superoxide dismutase and catalase activities (P < 0.01). Results of enzyme-linked immunosorbent assay (ELISA) showed that WEKPPVSH significantly mitigated the secretion of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) (P < 0.01). Immunofluorescence analysis exhibited that WEKPPVSH down-regulated p65 translocation to the cell nucleus. Western blotting showed that WEKPPVSH up-regulated the expression of nuclear factor erythroid 2-related factor (Nrf2) and heme oxygenase-1 (HO-1), and down-regulated the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), p-IκB/IκB, p-p65/p65 and p-p38/p38. In summary, WEKPPVSH might protect against oxidative stress and inflammation in LPS-stimulated BV-2 microglia by enhancing the Nrf2/HO-1 signaling pathway and blocking the nuclear factor-κB/p38 mitogen - activated protein kinase (NF-κB/p38 MAPK) signaling pathway. The results provided an experimental basis for the research and development of walnut peptide products.

• Authors: Yawen Gao, Hanxiong Qin, Dan Wu, Chunlei Liu, Li Fang, Ji Wang, Xiaoting Liu, Weihong Min
• Journal: Journal of bioscience and bioengineering (J Biosci Bioeng) Vol. 132 Issue 5 Pg. 496-504 (Nov 2021) ISSN: 1347-4421 [Electronic] Japan
• PMID: 34509368 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Chemical References

• Lipopolysaccharides
• NF-E2-Related Factor 2
• NF-kappa B
• Nitric Oxide
• Nitric Oxide Synthase Type II
• Heme Oxygenase-1
• p38 Mitogen-Activated Protein Kinases

Topics

• Heme Oxygenase-1 (genetics, metabolism)
• Inflammation
• Juglans (metabolism)
• Lipopolysaccharides (pharmacology)
• Microglia (metabolism)
• NF-E2-Related Factor 2 (genetics, metabolism)
• NF-kappa B (genetics, metabolism)
• Nitric Oxide (metabolism)
• Nitric Oxide Synthase Type II (genetics, metabolism)
• Oxidative Stress
• p38 Mitogen-Activated Protein Kinases (metabolism)