Lactobacillus plantarum lipoteichoic acid alleviates TNF-α-induced inflammation in the HT-29 intestinal epithelial cell line.

Abstract

We recently observed that lipoteichoic acid (LTA) isolated from Lactobacillus plantarum inhibited endotoxin-mediated inflammation of the immune cells and septic shock in a mouse model. Here, we examined the inhibitory role of L. plantarum LTA (pLTA) on the inflammatory responses of intestinal epithelial cells (IEC). The human colon cell line, HT-29, increased interleukin (IL)-8 expression in response to recombinant human tumor necrosis factor (TNF)-alpha, but not in response to bacterial ligands and interferon (IFN)-gamma. TNF-α also increased the production of inducible nitric oxide synthase (iNOS), nitric oxide (NO), and intercellular adhesion molecule 1 (ICAM-1) through activation of p38 mitogen-activated protein kinase (MAPK) from HT-29 cells. However, the inflammatory response of HT-29 on TNF-α stimulation was significantly inhibited by pLTA treatment. This pLTA-mediated inhibition accompanied the inhibition of nuclear factor (NF)-kappa B and MAPKs. Our data suggest that pLTA regulates cytokine-mediated immune responses and may be a good candidate for maintaining intestinal homeostasis against excessive inflammation.

• Authors: Hangeun Kim, Bong Jun Jung, Ji Hae Jung, Joo Yun Kim, Sung Kyun Chung, Dae Kyun Chung
• Journal: Molecules and cells (Mol Cells) Vol. 33 Issue 5 Pg. 479-86 (May 2012) ISSN: 0219-1032 [Electronic] United States
• PMID: 22526394 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-8
• Lipopolysaccharides
• NF-kappa B
• Teichoic Acids
• Tumor Necrosis Factor-alpha
• Intercellular Adhesion Molecule-1
• Nitric Oxide
• lipoteichoic acid
• Interferon-gamma
• Nitric Oxide Synthase Type II
• p38 Mitogen-Activated Protein Kinases

Topics

• Cell Adhesion (drug effects)
• Cell Line, Tumor
• Epithelial Cells (drug effects, metabolism, pathology)
• HT29 Cells
• Humans
• Inflammation (drug therapy, metabolism)
• Intercellular Adhesion Molecule-1 (metabolism)
• Interferon-gamma (metabolism)
• Interleukin-8 (antagonists & inhibitors, metabolism)
• Intestinal Mucosa (drug effects, metabolism, pathology)
• Intestines (drug effects, pathology)
• Lactobacillus plantarum (chemistry, metabolism)
• Lipopolysaccharides (pharmacology)
• NF-kappa B (antagonists & inhibitors, metabolism)
• Nitric Oxide (antagonists & inhibitors, metabolism)
• Nitric Oxide Synthase Type II (metabolism)
• Teichoic Acids (pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)
• p38 Mitogen-Activated Protein Kinases (antagonists & inhibitors, metabolism)