Characterizing T-cell phenotypes in nasal polyposis in Chinese patients.

Abstract

BACKGROUND:

Nasal polyposis has different etiologies in Western and Eastern countries. Furthermore, its pathogenesis is still poorly understood.

OBJECTIVE:

To determine the T-cell phenotypes involved in nasal polyposis in Chinese patients.

METHODS:

Twenty-four Chinese patients with nasal polyps were studied. CD4, CD8, Foxp3, and interleukin (IL) 17 were analyzed by immunohistochemical staining. Expression of T-bet, GATA-3, Foxp3, and RORgammat mRNA was detected by real-time polymerase chain reaction. The levels of T-cell cytokines (IL-4, IL-5, interferon [IFN] gamma, IL-10, IL-17, and transforming growth factor [TGF] beta) were determined using enzyme-linked immunosorbent assay, and serum immunoglobulin (Ig) E levels were measured using the UNICAP system.

RESULTS:

Increased expression of CD4+ and CD8+ and decreased expression of Foxp3 and IL-17 were detected in nasal polyps compared with control tissue. Furthermore, expression of T-bet and GATA-3 mRNA was upregulated, whereas Foxp3 mRNA expression was markedly downregulated. Furthermore, increased levels of IFN-gamma, IL-4 and IL-5 and decreased levels of IL-10 and TGF-beta were found in nasal polyps. There was no association between Staphylococcus aureus exotoxin (SAE)-specific IgE and T regulatory cell (Treg) insufficiency in nasal polyps.

CONCLUSIONS:

Our findings demonstrate that excessive infiltration of CD4+ and CD8+ T cells in nasal polyps may be associated with expression of Foxp3+ by Tregs but not with SAEs in Chinese patients.

• Authors: J Shi, Y Fan, R Xu, K Zuo, L Cheng, G Xu, H Li
• Journal: Journal of investigational allergology & clinical immunology (J Investig Allergol Clin Immunol) Vol. 19 Issue 4 Pg. 276-82 ( 2009) ISSN: 1018-9068 [Print] Spain
• PMID: 19639723 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD4 Antigens
• CD8 Antigens
• Cytokines
• FOXP3 protein, human
• Forkhead Transcription Factors
• GATA3 Transcription Factor
• T-Box Domain Proteins
• T-box transcription factor TBX21
• Transforming Growth Factor beta
• Immunoglobulin E

Topics

• Adult
• CD4 Antigens (biosynthesis)
• CD8 Antigens (biosynthesis)
• China
• Cytokines (immunology, metabolism)
• Female
• Forkhead Transcription Factors (genetics, immunology, metabolism)
• GATA3 Transcription Factor (genetics, immunology, metabolism)
• Humans
• Immunoglobulin E (blood)
• Immunophenotyping
• Male
• Middle Aged
• Nasal Polyps (blood, immunology, pathology, physiopathology)
• Rhinitis (blood, genetics, immunology, pathology, physiopathology)
• Sinusitis (blood, genetics, immunology, pathology, physiopathology)
• T-Box Domain Proteins (genetics, immunology, metabolism)
• T-Lymphocyte Subsets (immunology, metabolism, pathology)
• T-Lymphocytes, Regulatory (immunology, metabolism, pathology)
• Transforming Growth Factor beta (immunology, metabolism)