Abstract | PURPOSE: METHODS: RESULTS: NQO1 activity in KKU-OCA17 and HeLa Chang liver cells was suppressed by cytokine combination. Cytokines induced formation of NO and suppression of redox ratios of GSH and glutathione disulfide ( GSSG). GSNO produced the similar effects as cytokines on KKU-OCA17, in contrast, GSNO induced increase of NQO1 activity in HeLa Chang liver cells. The treatment of cytokines or GSNO suppressed expression of NQO1 in KKU-OCA17 and HeLa Chang liver cells. CONCLUSIONS: Inflammatory cytokines induced oxidative stress and this is associated with suppression of NQO1, whereas may contribute to differential susceptibility of biliary epithelial cells to chemical-induced cytotoxicity and carcinogenesis.
|
Authors | Auemduan Prawan, Benjaporn Buranrat, Upa Kukongviriyapan, Banchob Sripa, Veerapol Kukongviriyapan |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 135
Issue 4
Pg. 515-22
(Apr 2009)
ISSN: 1432-1335 [Electronic] Germany |
PMID | 18820947
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Superoxides
- Nitric Oxide
- NAD(P)H Dehydrogenase (Quinone)
- NQO1 protein, human
- Glutathione
- Glutathione Disulfide
|
Topics |
- Bile Duct Neoplasms
(genetics, pathology, physiopathology)
- Bile Ducts, Intrahepatic
(cytology, pathology, physiology)
- Cell Line, Tumor
- Cell Survival
- Cholangiocarcinoma
(genetics, pathology, physiopathology)
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Neoplastic
- Glutathione
(metabolism)
- Glutathione Disulfide
(metabolism)
- HeLa Cells
- Humans
- Inflammation
(physiopathology)
- Liver
(enzymology)
- NAD(P)H Dehydrogenase (Quinone)
(genetics)
- Nitric Oxide
(metabolism)
- Oxidative Stress
- Reverse Transcriptase Polymerase Chain Reaction
- Superoxides
(metabolism)
|