Abstract | BACKGROUND: METHODS: Forty-eight chronic periodontitis patients were randomly assigned to 2 groups (SRP plus subgingival application of vehicle control [SRP + V], or SRP plus subgingival application of minocycline microspheres [SRP + M]) and monitored at 8 sites per subject at baseline and 1, 3, and 6 months. Four shallow (PD < or = 3 mm) and 4 deep (PD > or = 5 mm) sites were evaluated for both marker levels and for probing depth (PD), clinical attachment level (CAL), and bleeding on probing (BOP). Eight periodontally healthy control subjects with no probing depths >3 mm and no loss of attachment were also monitored at the same time intervals. GCF levels of ICTP and IL-1 were determined using radioimmunoassay and enzyme-linked immunosorbent assay techniques, respectively. RESULTS: Significant differences (P<0.001) in GCF levels of ICTP and IL-1 were found between deep and shallow sites at all time points in both treatment groups. In addition, healthy subjects demonstrated significantly reduced levels of both markers compared to both shallow and deep sites in periodontitis patients (P <0.001). Only the SRP + M treated patients exhibited significant reductions (P <0.05) in both ICTP and IL-1 levels 1 month after treatment. Furthermore, the SRP + M group demonstrated significantly lower IL-1 levels (P <0.02) at 1 month compared to the SRP + V group. CONCLUSIONS: Results of this study indicate that GCF levels of ICTP and IL-1 correlate with clinical measures of periodontal disease and may aid in assessing disease status and response to periodontal therapy. Furthermore, local administration of minocycline microspheres led to a potent short-term reduction in GCF IL-1 levels. Additional studies are needed to address whether repeated administration of scaling and root planing along with minocycline microspheres will achieve long-term reductions in GCF ICTP and IL-1 levels.
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Authors | R J Oringer, K F Al-Shammari, W A Aldredge, V J Iacono, R M Eber, H L Wang, B Berwald, R Nejat, W V Giannobile |
Journal | Journal of periodontology
(J Periodontol)
Vol. 73
Issue 8
Pg. 835-42
(Aug 2002)
ISSN: 0022-3492 [Print] United States |
PMID | 12211491
(Publication Type: Clinical Trial, Comparative Study, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- Biomarkers
- Collagen Type I
- Interleukin-1
- Peptides
- collagen type I trimeric cross-linked peptide
- Collagen
- Minocycline
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Topics |
- Administration, Topical
- Adult
- Aged
- Analysis of Variance
- Anti-Bacterial Agents
(administration & dosage, therapeutic use)
- Biomarkers
(analysis)
- Bone Resorption
(drug therapy, therapy)
- Chronic Disease
- Collagen
(analysis, drug effects)
- Collagen Type I
- Dental Scaling
- Female
- Follow-Up Studies
- Gingival Crevicular Fluid
(chemistry)
- Gingival Hemorrhage
(drug therapy, therapy)
- Humans
- Interleukin-1
(analysis)
- Male
- Matched-Pair Analysis
- Microspheres
- Middle Aged
- Minocycline
(administration & dosage, therapeutic use)
- Peptides
(analysis, drug effects)
- Periodontal Attachment Loss
(drug therapy, therapy)
- Periodontal Pocket
(drug therapy, therapy)
- Periodontitis
(drug therapy, therapy)
- Root Planing
- Single-Blind Method
- Statistics as Topic
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