Bisacurone gel ameliorated burn wounds in experimental rats via its anti-inflammatory, antioxidant, and angiogenic properties.

Abstract

PURPOSE:

To investigate putative mechanism of wound healing for chitosan-based bisacurone gel against secondary burn wounds in rats.

METHODS:

A second-degree burn wound with an open flame using mixed fuel (2 mL, 20 seconds) was induced in Sprague Dawley rats (male, 180-220 g, n = 15, each) followed by topical treatments with either vehicle control (white petroleum gel, 1%), silver sulfadiazine (1%) or bisacurone gel (2.5, 5, or 10%) for 20 days. Wound contraction rate and paw withdrawal threshold were monitored on various days. Oxidative stress (superoxide dismutase, glutathione, malondialdehyde, and nitric oxide), pro-inflammatory cytokines (tumour necrosis factor-alpha, interleukins by enzyme-linked immunosorbent assay), growth factors (transforming growth factor-β, vascular endothelial growth factor C using real time polymerase chain reaction and Western blot assay) levels, and histology of wound skin were assessed at the end.

RESULTS:

Bisacurone gel showed 98.72% drug release with a 420.90-442.70 cps viscosity. Bisacurone gel (5 and 10%) significantly (p < 0.05) improved wound contraction rate and paw withdrawal threshold. Bisacurone gel attenuated oxidative stress, pro-inflammatory cytokines, and water content. It also enhanced angiogenesis (hydroxyproline and growth factor) and granulation in wound tissue than vehicle control.

CONCLUSIONS:

These findings suggested that bisacurone gel can be a potential candidate to treat burn wounds via its anti-inflammatory, antioxidant, and angiogenic properties.

• Authors: Zengqiang Yan, Shuyan Li, Zhenzhong Gong
• Journal: Acta cirurgica brasileira (Acta Cir Bras) Vol. 38 Pg. e382423 ( 2023) ISSN: 1678-2674 [Electronic] Brazil
• PMID: 37610964 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• bisacurone
• Vascular Endothelial Growth Factor C
• Cytokines
• Anti-Inflammatory Agents

Topics

• Male
• Rats
• Animals
• Antioxidants (pharmacology)
• Rats, Sprague-Dawley
• Vascular Endothelial Growth Factor C
• Cytokines
• Anti-Inflammatory Agents (pharmacology)