Zingiber roseum is a perennial herb in the Zingiberaceae family. The plant is native to Bangladesh, and rhizomes are frequently used in
traditional medicine to cure
gastric ulcers,
asthma,
wounds, and rheumatic disorders. Therefore, the present study aimed to analyse the
antipyretic, anti-inflammatory, and
analgesic properties of Z. roseum rhizome to confirm its efficacy in traditional applications. After 24 h of treatment, ZrrME (400 mg/kg) showed a considerable drop in rectal temperature (3.42°F) compared to standard
paracetamol (5.26°F). At both doses (200 and 400 mg/kg), ZrrME showed a substantial dose-dependent decrease in paw oedema. However, after 2, 3 and 4 h of testing, the extract (200 mg/kg) had a lower anti-inflammatory response than standard
indomethacin, whereas the higher dose (400 mg/kg) of rhizome extract had a more robust response compared to standard. ZrrME also showed substantial
analgesic activity against all in vivo
analgesic test models. The in vivo findings were further evaluated by in silico study of our previously identified compounds of ZrrME with the
cyclooxygenase-2 enzyme (3LN1). The substantial binding energy (ranges from-6.2 to-7.7 Kcal/mol) of the
polyphenols (excluding
catechin hydrate) to the COX-2
enzyme affirm the in vivo test results of the present studies. In addition, the compounds were found effective as
antipyretic, anti-inflammatory, and
analgesic agents, according to the
biological activity prediction software. Both in vivo and in silico results demonstrated promising
antipyretic, anti-inflammatory, and
pain-relieving effects of Z. roseum rhizome extract, which corroborate the claim of its traditional uses.