Disposable immunoplatforms for the simultaneous determination of biomarkers for neurodegenerative disorders using poly(amidoamine) dendrimer/gold nanoparticle nanocomposite.

Abstract

Early diagnosis in primary care settings can increase access to therapies and their efficiency as well as reduce health care costs. In this context, we report in this paper the development of a disposable immunoplatform for the rapid and simultaneous determination of two protein biomarkers recently reported to be involved in the pathological process of neurodegenerative disorders (NDD), tau protein (tau), and TAR DNA-binding protein 43 (TDP-43). The methodology involves implementation of a sandwich-type immunoassay on the surface of dual screen-printed carbon electrodes (dSPCEs) electrochemically grafted with p-aminobenzoic acid (p-ABA), which allows the covalent immobilization of a gold nanoparticle-poly(amidoamine) (PAMAM) dendrimer nanocomposite (3D-Au-PAMAM). This scaffold was employed for the immobilization of the capture antibodies (CAbs). Detector antibodies labeled with horseradish peroxidase (HRP) and amperometric detection at - 0.20 V (vs. Ag pseudo-reference electrode) using the H2O2/hydroquinone (HQ) system were used. The developed methodology exhibits high sensitivity and selectivity for determining the target proteins, with detection limits of 2.3 and 12.8 pg mL-1 for tau and TDP-43, respectively. The simultaneous determination of tau and TDP-43 was accomplished in raw plasma samples and brain tissue extracts from healthy individuals and NDD-diagnosed patients. The analysis can be performed in just 1 h using a simple one-step assay protocol and small sample amounts (5 μL plasma and 2.5 μg brain tissue extracts). Graphical abstract.

• Authors: Verónica Serafín, Claudia A Razzino, Maria Gamella, María Pedrero, Eloy Povedano, Ana Montero-Calle, Rodrigo Barderas, Miguel Calero, Anderson O Lobo, Paloma Yáñez-Sedeño, Susana Campuzano, José M Pingarrón
• Journal: Analytical and bioanalytical chemistry (Anal Bioanal Chem) Vol. 413 Issue 3 Pg. 799-811 (Jan 2021) ISSN: 1618-2650 [Electronic] Germany
• PMID: 32474723 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• DNA-Binding Proteins
• Dendrimers
• MAPT protein, human
• Poly(amidoamine)
• Polyamines
• TARDBP protein, human
• tau Proteins
• Gold

Topics

• Biomarkers (blood, metabolism)
• Brain (metabolism)
• Case-Control Studies
• DNA-Binding Proteins (blood, metabolism)
• Dendrimers (chemistry)
• Electrodes
• Gold (chemistry)
• Humans
• Immunoassay (methods)
• Metal Nanoparticles (chemistry)
• Neurodegenerative Diseases (blood, diagnosis, metabolism)
• Polyamines (chemistry)
• tau Proteins (blood, metabolism)