Hesperidin Prevents Nitric Oxide Deficiency-Induced Cardiovascular Remodeling in Rats via Suppressing TGF-β1 and MMPs Protein Expression.

Abstract

Hesperidin is a major flavonoid isolated from citrus fruits that exhibits several biological activities. This study aims to evaluate the effect of hesperidin on cardiovascular remodeling induced by n-nitro l-arginine methyl ester (l-NAME) in rats. Male Sprague-Dawley rats were treated with l-NAME (40 mg/kg), l-NAME plus hesperidin (15 mg/kg), hesperidin (30 mg/kg), or captopril (2.5 mg/kg) for five weeks (n = 8/group). Hesperidin or captopril significantly prevented the development of hypertension in l-NAME rats. l-NAME-induced cardiac remodeling, i.e., increases in wall thickness, cross-sectional area (CSA), and fibrosis in the left ventricular and vascular remodeling, i.e., increases in wall thickness, CSA, vascular smooth muscle cells, and collagen deposition in the aorta were attenuated by hesperidin or captopril. These were associated with reduced oxidative stress markers, tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta 1 (TGF-β1), and enhancing plasma nitric oxide metabolite (NOx) in l-NAME treated groups. Furthermore, up-regulation of tumor necrosis factor receptor type 1 (TNF-R1) and TGF- β1 protein expression and the overexpression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) was suppressed in l-NAME rats treated with hesperidin or captopril. These data suggested that hesperidin had cardioprotective effects in l-NAME hypertensive rats. The possible mechanism may involve antioxidant and anti-inflammatory effects.

• Authors: Putcharawipa Maneesai, Sarawoot Bunbupha, Prapassorn Potue, Thewarid Berkban, Upa Kukongviriyapan, Veerapol Kukongviriyapan, Parichat Prachaney, Poungrat Pakdeechote
• Journal: Nutrients (Nutrients) Vol. 10 Issue 10 (Oct 19 2018) ISSN: 2072-6643 [Electronic] Switzerland
• PMID: 30347737 (Publication Type: Journal Article)

Chemical References

• Transforming Growth Factor beta1
• Nitric Oxide
• Captopril
• Hesperidin
• Matrix Metalloproteinases
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Blood Pressure (drug effects)
• Captopril (pharmacology)
• Gene Expression Regulation (drug effects)
• Hesperidin (pharmacology)
• Male
• Matrix Metalloproteinases (genetics, metabolism)
• NG-Nitroarginine Methyl Ester (pharmacology)
• Nitric Oxide (metabolism, pharmacology)
• Rats
• Rats, Sprague-Dawley
• Transforming Growth Factor beta1 (genetics, metabolism)
• Vascular Remodeling (drug effects, physiology)
• Ventricular Remodeling (drug effects)