Interleukin-10 modulates pulmonary neutrophilic inflammation induced by cigarette smoke exposure.

Abstract

AIM OF THE STUDY:

Interleukin (IL)-10 is an anti-inflammatory cytokine, but its role in cigarette smoke (CS)-induced inflammation and chronic obstructive pulmonary disease (COPD) has not been fully elucidated. The purpose of this study was to investigate the effect of IL-10 deficiency on CS-induced pulmonary inflammation in mice in vivo and in vitro.

MATERIALS AND METHODS:

IL-10-deficient and wild-type control mice with a C57BL6/J genetic background were exposed to CS, and inflammatory cells in bronchoalveolar lavage fluid (BALF) and mRNA of cytokines in lung were evaluated with enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR).

RESULTS:

During 12 days of daily CS exposure to wild-type mice, neutrophil counts in BAL fluid and tumor necrosis factor (TNF)-α mRNA expression were increased, peaked at day 8, and then declined on day 12 when the level of IL-10 reached its peak. In IL-10-deficient mice, neutrophil recruitment and TNF-α mRNA levels induced by CS exposure were significantly greater than those in wild-type mice. Keratinocyte-derived chemokine (KC; murine ortholog of human CXCL8) and granulocyte macrophage colony-stimulating factor (GM-CSF) mRNA levels or matrix metalloproteinase(MMP)-9 protein levels were not correlated with neutrophil count.

CONCLUSIONS:

IL-10 had a modulatory effect on CS-induced pulmonary neutrophilic inflammation and TNF-α expression in mice in vivo and therefore appears to be an important endogenous suppressor of airway neutrophilic inflammation.

• Authors: Manabu Higaki, Hiroo Wada, Shinichiro Mikura, Tetsuo Yasutake, Masuo Nakamura, Mamoru Niikura, Fumie Kobayashi, Hiroshi Kamma, Shigeru Kamiya, Kazuhiro Ito, Peter J Barnes, Hajime Goto, Hajime Takizawa
• Journal: Experimental lung research (Exp Lung Res) Vol. 41 Issue 10 Pg. 525-34 ( 2015) ISSN: 1521-0499 [Electronic] England
• PMID: 26651880 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• IL10 protein, mouse
• Lipopolysaccharides
• Smoke
• Interleukin-10
• Matrix Metalloproteinase 9
• Mmp9 protein, mouse

Topics

• Animals
• Interleukin-10 (physiology)
• Lipopolysaccharides (toxicity)
• Macrophages, Peritoneal (drug effects)
• Male
• Matrix Metalloproteinase 9 (analysis)
• Mice
• Mice, Inbred C57BL
• Neutrophil Infiltration
• Pneumonia (etiology, pathology)
• Smoke (adverse effects)
• Nicotiana (adverse effects)