Green tea polyphenols ameliorate pancreatic injury in cerulein-induced murine acute pancreatitis.

Abstract

OBJECTIVE:

Green tea polyphenols (GTPs) are naturally occurring antioxidants acting through pathways that include reactive oxygen species and nuclear factor kappa B (NF-kappaB). This study investigates the effect of GTPs in a cerulein-induced murine model of acute pancreatitis (AP).

METHODS:

Male CD mice (median weight, 37.7 g) were divided into 4 groups: mice administered with cerulein alone, cerulein and GTP, saline alone (sham), and GTP alone. Acute pancreatitis was induced by serial intraperitoneal administration of cerulein (50 microg/kg, x6). Green tea polyphenol was administered intraperitoneally at 25 mg/kg on the first, third, and sixth hours after pancreatitis induction.We analyzed histologic and biochemical features of AP, NF-kappaB pathway activity, leukocyte-mediated damage, cytokine levels, oxidative stress injury, lipid peroxidation, expression of poly-(adenosine diphosphate-ribose) synthetase, and presence of apoptosis.

RESULTS:

Treatment with GTP reduced the histologic and biochemical features of AP. Western blot revealed significant NF-kappaB inactivation. Immunostaining for P selectin and intercellular adhesion molecule 1, tumor necrosis factor alpha, transforming growth factor beta, vascular endothelial growth factor, nitrotirosine, poly-(adenosine diphosphate ribose) synthetase, and malondialdheide levels were significantly reduced. There was a significant down-regulation of apoptotic markers.

CONCLUSIONS:

Our results demonstrated that GTP significantly ameliorated the effects of cerulein-induced AP in mice. These effects of GTP are mediated by actions at the NF-kappaB/IkB (inhibitor kB) proteins and oxidative stress pathways.

• Authors: Benoy I Babu, Giuseppe Malleo, Tiziana Genovese, Emanuela Mazzon, Rosanna Di Paola, Concetta Crisafulli, Rocco Caminiti, Ajith K Siriwardena, Salvatore Cuzzocrea
• Journal: Pancreas (Pancreas) Vol. 38 Issue 8 Pg. 954-67 (Nov 2009) ISSN: 1536-4828 [Electronic] United States
• PMID: 19672210 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• I-kappa B Proteins
• NF-kappa B
• P-Selectin
• Tea
• Transforming Growth Factor beta
• Tumor Necrosis Factor-alpha
• Vascular Endothelial Growth Factor A
• Intercellular Adhesion Molecule-1
• Phenol
• Ceruletide
• Poly(ADP-ribose) Polymerases

Topics

• Acute Disease
• Animals
• Apoptosis (drug effects)
• Blotting, Western
• Ceruletide
• I-kappa B Proteins (metabolism)
• Immunohistochemistry
• In Situ Nick-End Labeling
• Injections, Intraperitoneal
• Intercellular Adhesion Molecule-1 (metabolism)
• Lipid Peroxidation (drug effects)
• Male
• Mice
• NF-kappa B (metabolism)
• Neutrophil Infiltration (drug effects)
• P-Selectin (metabolism)
• Pancreas (drug effects, metabolism, pathology)
• Pancreatitis (chemically induced, prevention & control)
• Phenol (administration & dosage, pharmacology)
• Poly(ADP-ribose) Polymerases (metabolism)
• Tea (chemistry)
• Time Factors
• Transforming Growth Factor beta (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)