Abstract | RATIONALE: Increased oxidative stress and decreased superoxide dismutase (SOD) activity in the asthmatic airway are correlated to airflow limitation and hyperreactivity. We hypothesized that asthmatic individuals with higher levels of oxidative stress may have greater loss of SOD activity, which would be reflected systemically in loss of circulating SOD activity and clinically by development of severe asthma and/or worsening airflow limitation. METHODS: RESULTS: SOD activity, but not Mn-SOD or Cu,Zn-SOD protein, was lower in asthmatic serum as compared with control, and activity loss was significantly related to airflow limitation. Further, serum SOD activity demonstrated an inverse correlation with circulating levels of 3-bromotyrosine, a posttranslational modification of proteins produced by the eosinophil peroxidase system of eosinophils. Exposure of purified Cu,Zn-SOD to physiologically relevant levels of eosinophil peroxidase-generated reactive brominating species, reactive nitrogen species, or tyrosyl radicals in vitro confirmed that eosinophil-derived oxidative pathways promote enzyme inactivation. CONCLUSION: These findings are consistent with greater oxidant stress in asthma leading to greater inactivation of SOD, which likely amplifies inflammation and progressive airflow obstruction.
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Authors | Suzy A A Comhair, Kristin S Ricci, Mercedes Arroliga, Abigail R Lara, Raed A Dweik, Wei Song, Stanley L Hazen, Eugene R Bleecker, William W Busse, Kian Fan Chung, Benjamin Gaston, Annette Hastie, Mark Hew, Nizar Jarjour, Wendy Moore, Stephen Peters, W Gerald Teague, Sally E Wenzel, Serpil C Erzurum |
Journal | American journal of respiratory and critical care medicine
(Am J Respir Crit Care Med)
Vol. 172
Issue 3
Pg. 306-13
(Aug 01 2005)
ISSN: 1073-449X [Print] United States |
PMID | 15883124
(Publication Type: Journal Article, Multicenter Study, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Amino Acids
- Glutathione Peroxidase
- Superoxide Dismutase
- Glutathione
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Topics |
- Adult
- Airway Obstruction
(enzymology)
- Amino Acids
(blood)
- Asthma
(enzymology, metabolism)
- Case-Control Studies
- Female
- Forced Expiratory Volume
- Glutathione
(blood)
- Glutathione Peroxidase
(blood)
- Humans
- Male
- Oxidative Stress
- Respiratory Function Tests
- Spirometry
- Superoxide Dismutase
(blood, deficiency)
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