Abstract | BACKGROUND: OBJECTIVE: STUDY DESIGN:
Endometriosis was induced in 18 apolipoprotein E-null mice, the standard murine model used to study atherosclerosis. Mice of the same strain were used as controls (n=18) and underwent sham surgery without inducing endometriosis. The formation of endometriotic lesions was confirmed after 25 weeks of induction. Atherosclerotic lesions were subjected to hematoxylin and eosin staining followed by measurement of the aortic root luminal area and wall thickness. The whole aorta was isolated, and Oil Red O staining was performed to quantify the lipid deposits or plaque formation; moreover, biochemical assays were carried out in serum to determine the levels of lipids and inflammatory-related cytokines. RESULTS: CONCLUSION:
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Authors | Ramanaiah Mamillapalli, Nikoletta Toffoloni, Shutaro Habata, Huang Qunhua, Rula Atwani, Nina Stachenfeld, Hugh S Taylor |
Journal | American journal of obstetrics and gynecology
(Am J Obstet Gynecol)
Vol. 227
Issue 2
Pg. 248.e1-248.e8
(08 2022)
ISSN: 1097-6868 [Electronic] United States |
PMID | 35351413
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2022 Elsevier Inc. All rights reserved. |
Chemical References |
- Interleukin-1alpha
- Interleukin-6
- Vascular Endothelial Growth Factor A
- Interferon-gamma
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Topics |
- Animals
- Atherosclerosis
- Cardiovascular Diseases
- Disease Models, Animal
- Endometriosis
- Female
- Humans
- Inflammation
(metabolism)
- Interferon-gamma
- Interleukin-1alpha
- Interleukin-6
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Plaque, Atherosclerotic
- Vascular Endothelial Growth Factor A
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