Picroside II Improves Severe Acute Pancreatitis-Induced Hepatocellular Injury in Rats by Affecting JAK2/STAT3 Phosphorylation Signaling.

Abstract

Picroside II is an important ingredient agent in Traditional Chinese medicine and hoped to reduce hepatocellular injury caused by severe acute pancreatitis (SAP). An SAP-induced hepatocellular injury model was established in rats by using pentobarbital sodium. 27 rats were divided into 3 groups: the sham group (SG), model group (MG), and Picroside groups (PG). SAP-induced hepatocellular injury was assessed using hematoxylin and eosin staining. We measured hepatocellular enzymes (amylase (AMY), alanine aminotransferase (ALT), and aspartate aminotransferase (AST)), oxidative stress factors (superoxidase dismutase (SOD) and malondialdehyde (MDA)), and inflammatory factors (tumor necrosis factor α (TNF-α), interleukin- (IL-) 6, and IL-10), apoptotic factors (BAX and cleaved caspase 3), and inflammatory signaling (Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3), p-JAK2, and p-STAT3) in hepatocellular tissues. The SAP-induced hepatocellular injury model was successfully established. Picroside II treatment repaired hepatocellular injury by reducing the activities of AMY, ALT, and AST; reducing the levels of MDA, TNF-α, IL-1, IL-6, p-JAK2, p-STAT3, BAX, and cleaved caspase 3; and increasing the levels of SOD and IL-10. Picroside II exerted protective function for the SAP-induced hepatocellular injury model. Picroside II improved SAP-induced hepatocellular injury and antioxidant and anti-inflammatory properties by affecting JAK2/STAT3 phosphorylation signaling.

• Authors: Xuehua Piao, Xiaodan Sui, Baohai Liu, Tingfang Cui, Zinan Qi
• Journal: BioMed research international (Biomed Res Int) Vol. 2021 Pg. 9945149 ( 2021) ISSN: 2314-6141 [Electronic] United States
• PMID: 34368363 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Xuehua Piao et al.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Bile Acids and Salts
• Cinnamates
• Cytokines
• Iridoid Glucosides
• RNA, Messenger
• STAT3 Transcription Factor
• picroside II
• Janus Kinase 2

Topics

• Acute Disease
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (metabolism)
• Apoptosis (drug effects)
• Bile Acids and Salts (blood)
• Cholestasis (pathology)
• Cinnamates (chemistry, pharmacology)
• Cytokines (metabolism)
• Hepatocytes (drug effects, pathology)
• Iridoid Glucosides (chemistry, pharmacology)
• Janus Kinase 2 (metabolism)
• Liver (enzymology, pathology)
• Male
• Models, Biological
• Pancreatitis (blood, pathology)
• Phosphorylation (drug effects)
• RNA, Messenger (genetics, metabolism)
• Rats, Sprague-Dawley
• STAT3 Transcription Factor (metabolism)
• Signal Transduction