Abstract | INTRODUCTION: METHODS: Samples were collected from 21 root canals, and macrophages were stimulated for 24 hours. Tumor necrosis factor alpha (TNF-α), interleukin (IL)-6, IL-10, IL-12p70, interferon gamma, and chemokine (C-C motif) ligand 2 (CCL2) were measured using cytometric bead array. We investigated the overlapping networks between cytokines and chemokines with regression analysis. Checkerboard DNA- DNA hybridization was used to assess 40 target bacteria species. Using factor analysis, bacterial species aggregated in 2 factors. The association of bacteria species-based factors on cytokine and chemokine levels and clinical features was estimated with regression analysis. RESULTS: A negative relationship between IL-10 (anti-inflammatory cytokine) and CCL2, TNF-α, and IFN-γ (proinflammatory cytokines) (all P < .05) was observed. CCL2 was positively correlated with TNF-α (P < .01). Thirty-eight bacteria species were detected in primary endodontic infection with apical periodontitis. The first bacteria species-based factor was associated with the size of the radiolucent area (coefficient = 15.42) and tenderness to percussion/ pain on palpation (coefficient = 20.79). The second factor was associated with CCL2 levels (coefficient = 1.28). CONCLUSIONS: Different bacterial profiles can be differentially related to the expression of inflammatory proteins and the experience of clinical features.
|
Authors | Frederico C Martinho, Fabio R M Leite, Rodrigo Arruda-Vasconcelos, Lidiane M Louzada, Richard P Darveau, Brenda P F A Gomes |
Journal | Journal of endodontics
(J Endod)
Vol. 47
Issue 8
Pg. 1265-1271
(Aug 2021)
ISSN: 1878-3554 [Electronic] United States |
PMID | 34015415
(Publication Type: Journal Article)
|
Copyright | Copyright © 2021 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Chemokine CCL2
- Cytokines
- Interleukin-6
- Tumor Necrosis Factor-alpha
|
Topics |
- Bacteria
- Chemokine CCL2
- Cytokines
- Humans
- Interleukin-6
- Periapical Periodontitis
- Tumor Necrosis Factor-alpha
|