Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: Sphagneticola trilobata (L.) Pruski is a plant species belonging to the Asteraceae family. Kaurenoid acid (KA) is a diterpene metabolite and one of the active ingredients of Sphagneticola trilobata (L.) Pruski. Extracts containing KA are used in traditional medicine to treat pain, inflammation, and infection. AIM: The goal of the present study was to investigate the in vivo effects of KA (1-10 mg/kg, per oral gavage) upon LPS inoculation in mice by intraperitoneal (i.p.) or intraplantar (i.pl.; subcutaneous plantar injection) routes at the dose of 200 ng (200 μL or 25 μL, respectively). METHODS: RESULTS: KA inhibited LPS-induced mechanical and thermal hyperalgesia, MPO activity and modulated redox status in the mice paw. Pre- and post-treatment with KA inhibited migration of neutrophils and monocytes in LPS peritonitis. KA inhibited the pro-inflammatory/hyperalgesic cytokine (e.g., TNF-α, IL-1β and IL-33) production while enhanced anti-inflammatory/ analgesic cytokine IL-10 in peritoneal cavity. In agreement with the effect of KA over pro-inflammatory cytokines it inhibited oxidative stress (total ROS, superoxide production and superoxide positive cells) and NF-κB activation during peritonitis. CONCLUSION:
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Authors | Sergio M Borghi, Sandra S Mizokami, Thacyana T Carvalho, Fernanda S Rasquel-Oliveira, Camila R Ferraz, Victor Fattori, Thiago H Hayashida, Jean P S Peron, Doumit Camilios-Neto, Sergio R Ambrosio, Nilton S Arakawa, Rubia Casagrande, Waldiceu A Verri Jr |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 273
Pg. 113980
(Jun 12 2021)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 33652112
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Elsevier B.V. All rights reserved. |
Chemical References |
- Analgesics
- Diterpenes
- Lipopolysaccharides
- NF-kappa B
- kaurenoic acid
- Peroxidase
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Topics |
- Analgesics
(chemistry, therapeutic use)
- Animals
- Asteraceae
(chemistry)
- Diterpenes
(chemistry, therapeutic use)
- Gene Expression Regulation
(drug effects)
- Hyperalgesia
(drug therapy)
- Lipid Peroxidation
- Lipopolysaccharides
(toxicity)
- Male
- Mice
- Molecular Structure
- NF-kappa B
(genetics, metabolism)
- Pain
(drug therapy)
- Peritonitis
(chemically induced, drug therapy)
- Peroxidase
(metabolism)
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