Abstract | Background: Methods: The PCSK9 was compared between GO (n=11) and normal subjects (n=7) in orbital tissue explants using quantitative real-time PCR, and in cultured interleukin-1β (IL-1β)-treated fibroblasts using western blot. Western blot was used to identify the effects of PCSK9 inhibition on IL-1β-induced pro-inflammatory cytokines production and signaling molecules expression as well as levels of adipogenic markers and oxidative stress-related proteins. Adipogenic differentiation was identified using Oil Red O staining. The plasma PCSK9 concentrations were compared between patients with GO (n=44) and healthy subjects (n=26) by ELISA. Results: Conclusions: PCSK9 plays a significant role in GO. The PCSK9 inhibition attenuated the pro-inflammatory cytokines production, oxidative stress, and fibroblast differentiation into adipocytes. PCSK9 may serve as a therapeutic target and biomarker for GO.
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Authors | Ga Eun Lee, Jinjoo Kim, Jihei Sara Lee, JaeSang Ko, Eun Jig Lee, Jin Sook Yoon |
Journal | Frontiers in endocrinology
(Front Endocrinol (Lausanne))
Vol. 11
Pg. 607144
( 2020)
ISSN: 1664-2392 [Print] Switzerland |
PMID | 33488522
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021 Lee, Kim, Lee, Ko, Lee and Yoon. |
Chemical References |
- Cell Adhesion Molecules
- Cytokines
- IL1B protein, human
- Interleukin-1beta
- PCSK9 protein, human
- Proprotein Convertase 9
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Topics |
- Adipocytes
- Adipogenesis
(genetics)
- Adult
- Cell Adhesion Molecules
(metabolism)
- Cell Differentiation
(genetics)
- Cells, Cultured
- Cytokines
(metabolism)
- Female
- Fibroblasts
(metabolism)
- Graves Ophthalmopathy
(genetics, metabolism)
- Humans
- Interleukin-1beta
(pharmacology)
- Male
- Middle Aged
- Orbit
(cytology)
- Oxidative Stress
(genetics)
- Proprotein Convertase 9
(drug effects, genetics, metabolism)
- Young Adult
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