Roflumilast prevents lymphotoxin α (TNF-β)-induced inflammation activation and degradation of type 2 collagen in chondrocytes.

Abstract

BACKGROUND AND PURPOSE:

Osteoarthritis (OA) is a chronic disease accompanied by severe inflammation. The inflammation activation in the chondrocytes and the degradation of the extracellular matrix were reported to be involved in the progress of OA. Roflumilast is a selective PDE4 inhibitor used for treating chronic obstructive pulmonary disease (COPD) and exerts significant anti-inflammation effects. The present study aims to investigate the effects of Roflumilast on tumor necrosis factor-β (TNF-β)-induced inflammation activation and degradation of type 2 collagen in chondrocytes.

METHODS:

TNF-β was used to establish the in-vitro inflammation model on ATDC5 chondrocytes. Quantitative real-time polymerase chain reaction (QRT-PCR) and western blot were used to determine the expression level of tumor necrosis factor receptor 2 (TNFR2), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), matrix metalloproteinase 3 (MMP-3), matrix metalloproteinase 13 (MMP-13), type 2 collagen and nuclear factor kappa B (NF-κB) p65. The release of prostaglandin E2 (PGE2), MMP-3, and MMP-13 were evaluated by ELISA. The production of NO was determined by DAF-FM DA staining and the function of the NF-κB promoter was evaluated by Luciferase activity assay.

RESULTS:

TNFR2 and COX-2 were upregulated and the release of PGE2 was promoted by TNF-β stimulation, which were all inhibited by Roflumilast. Roflumilast suppressed the promoted iNOS expression and NO production induced by TNF-β. MMP-3 and MMP-13 were up-regulated, and type 2 collagen was down-regulated by TNF-β stimulation, which were all reversed by Roflumilast. Roflumilast inhibited the promoted releasing of Interleukin-8 (IL-8) and Interleukin-12 (IL-12), expression of up-regulated NF-κB, and activation of NF-κB transcriptional activity induced by TNF-β.

CONCLUSION:

Roflumilast may prevent TNF-β-induced inflammation activation and degradation of type 2 collagen in chondrocytes.

• Authors: Jingtong Zhao, Lian Duan, Renxiang Wang, Yi Liu, Jinlan Jiang
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 69 Issue 12 Pg. 1191-1199 (Dec 2020) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 32990777 (Publication Type: Journal Article)

Chemical References

• Aminopyridines
• Benzamides
• Collagen Type II
• Cyclopropanes
• Cytokines
• Lymphotoxin-alpha
• Phosphodiesterase 4 Inhibitors
• Receptors, Tumor Necrosis Factor, Type II
• Roflumilast

Topics

• Aminopyridines (pharmacology)
• Benzamides (pharmacology)
• Cell Line
• Chondrocytes (drug effects, metabolism)
• Collagen Type II (metabolism)
• Cyclopropanes (pharmacology)
• Cytokines (metabolism)
• Extracellular Matrix (drug effects)
• Humans
• Inflammation (chemically induced, prevention & control)
• Lymphotoxin-alpha
• Phosphodiesterase 4 Inhibitors (pharmacology)
• Receptors, Tumor Necrosis Factor, Type II (metabolism)
• Signal Transduction (drug effects)