Abstract | AIM OF STUDY:
Cholangiocarcinoma (CCA) is an aggressive cancer with considerable metastatic potential. Various cytokines secreted by tumor cells or cells in the tumor environment can promote the metastasis of CCA. The aim of the present study was to investigate the effect of myricetin on the inhibition of cytokine-induced migration and invasion and the associated cellular mechanisms in human CCA cells. MATERIALS AND METHODS: CCA KKU-100 cells were treated with a pro-inflammatory cytokine mixture consisting of interleukin-6, interferon-γ, and tumor necrosis factor-α. The migratory and invasive ability of KKU-100 cells were determined using a wound-healing assay and transwell invasion assay. The effect of myricetin on cytokine-induced STAT3 activation in CCA cells was determined using Western blot analysis. The real-time polymerase chain reaction was performed to determine messenger RNA expression. RESULTS: CONCLUSION: This finding reveals the anti-metastatic effect of myricetin against CCA cells which is mediated partly through suppression of the STAT3 pathway. This compound could be potentially useful as a therapeutic agent against CCA.
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Authors | Pattarapon Tuponchai, Veerapol Kukongviriyapan, Auemduan Prawan, Sarinya Kongpetch, Laddawan Senggunprai |
Journal | Journal of cancer research and therapeutics
(J Cancer Res Ther)
2019 Jan-Mar
Vol. 15
Issue 1
Pg. 157-163
ISSN: 1998-4138 [Electronic] India |
PMID | 30880773
(Publication Type: Journal Article)
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Chemical References |
- Cytokines
- Flavonoids
- STAT3 Transcription Factor
- STAT3 protein, human
- myricetin
- Indomethacin
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Topics |
- Bile Duct Neoplasms
(drug therapy, pathology)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cholangiocarcinoma
(drug therapy, pathology)
- Cytokines
(metabolism)
- Drug Screening Assays, Antitumor
- Flavonoids
(pharmacology, therapeutic use)
- Humans
- Indomethacin
(pharmacology, therapeutic use)
- Neoplasm Invasiveness
(pathology, prevention & control)
- STAT3 Transcription Factor
(metabolism)
- Signal Transduction
(drug effects)
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