Abstract | OBJECTIVES: This study aimed to assess the biological impact of occupational exposure to diesel exhaust (DE) including DE particles ( DEP) from heavy-duty diesel-powered equipment in Norwegian tunnel finishing workers (TFW). METHODS: TFW (n=69) and referents (n=69) were investigated for bulky DNA adducts (by 32P-postlabelling) and expression of microRNAs ( miRNAs) (by small RNA sequencing) in peripheral blood mononuclear cells (PBMC), as well as circulating free arachidonic acid (AA) and eicosanoid profiles in plasma (by liquid chromatography-tandem mass spectrometry). RESULTS: CONCLUSION: Occupational exposure to DE/ DEP is associated with biological alterations in TFW potentially affecting lung homoeostasis, carcinogenesis, inflammation status and the cardiovascular system. Of particular importance is the finding that tunnel finishing work is associated with an increased level of DNA adducts formation in PBMC.
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Authors | Iselin Rynning, Volker M Arlt, Kristyna Vrbova, Jiří Neča, Pavel Rossner Jr, Jiri Klema, Bente Ulvestad, Elisabeth Petersen, Øivind Skare, Aage Haugen, David H Phillips, Miroslav Machala, Jan Topinka, Steen Mollerup |
Journal | Occupational and environmental medicine
(Occup Environ Med)
Vol. 76
Issue 1
Pg. 10-16
(01 2019)
ISSN: 1470-7926 [Electronic] England |
PMID | 30425118
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. |
Chemical References |
- Air Pollutants, Occupational
- Biomarkers
- DNA Adducts
- Lipids
- MicroRNAs
- Vehicle Emissions
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Topics |
- Adult
- Air Pollutants, Occupational
(analysis)
- Biomarkers
(blood)
- Construction Industry
- Cross-Sectional Studies
- DNA Adducts
(blood)
- Humans
- Inhalation Exposure
(analysis)
- Leukocytes, Mononuclear
(chemistry)
- Linear Models
- Lipids
(blood)
- Male
- MicroRNAs
(blood)
- Middle Aged
- Norway
- Occupational Exposure
(adverse effects)
- Vehicle Emissions
(toxicity)
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