Painful diabetic neuropathy (PDN) is a common and troublesome
diabetes complication.
Protein kinase C (PKC)-mediated dorsal root ganglia (DRG)
P2X3 receptor upregulation is one important mechanism underlying PDN. Accumulating evidence demonstrated that
electroacupuncture (EA) at low frequency could effectively attenuate
neuropathic pain. Our previous study showed that 2-Hz EA could relieve
pain well in PDN. The study aimed to investigate whether 2-Hz EA relieves
pain in PDN through suppressing PKC-mediated DRG
P2X3 receptor upregulation. A 7-week feeding of high-fat and high-
sugar diet plus a single injection of
streptozotocin (STZ) in a dose of 35 mg/kg after a 5-week feeding of the diet successfully induced type 2 PDN in rats as revealed by the elevated
body weight, fasting
blood glucose, fasting
insulin and
insulin resistance, and the reduced paw withdrawal threshold (PWT), as well as the destructive ultrastructural change of sciatic nerve. DRG plasma membrane
P2X3 receptor level and DRG PKC expression were elevated. Two-hertz EA failed to improve
peripheral neuropathy; however, it reduced PWT, DRG plasma membrane
P2X3 receptor level, and DRG PKC expression in PDN rats. Intraperitoneal administration of
P2X3 receptor agonist αβ-meATP or PKC activator
phorbol 12-myristate 13-acetate (PMA) blocked 2-Hz EA
analgesia. Furthermore, PMA administration increased DRG plasma membrane
P2X3 receptor level in PDN rats subject to 2-Hz EA treatment. These findings together indicated that the
analgesic effect of EA in PDN is mediated by suppressing PKC-dependent membrane P2X3 upregulation in DRG. EA at low frequency is a valuable approach for PDN control.