Role of IL-1ra and Granzyme B as biomarkers in active Crohn's disease patients.

Abstract

BACKGROUND AND AIM:

Altered gene expression in intestinal mucosa is thought to contribute to inflammatory process in Crohn's disease (CD). The present study investigated changes in the expression of genes associated with gut inflammation in CD patients by RNA microarray to identify disease biomarkers.

METHODS:

Microarray analysis was carried out in formalin-fixed, paraffin-embedded intestinal tissue specimens from six CD patients who underwent surgery without prior treatment and from two healthy control subjects. Transcripts overexpressed in CD patients were validated by enzyme-linked immunosorbent assay (ELISA) using specimens from 46 CD patients and 60 healthy controls.

RESULTS:

Among the genes over-expressed with statistical significance, five genes including decay-accelerating factor, interleukin-1 receptor (IL1R) A, tumour necrosis factor receptor 2, (C-X-C motif) ligand (CXCL) 1, and granzyme (GZM) B proposed to have functional association with CD were selected to validate the expressed transcripts in serum. Serum concentration of IL1RA, CXCL1, and GZMB measured by ELISA were significantly higher in CD patients.

CONCLUSIONS:

We identified that IL1RA, CXCL1, and GZMB are overexpressed in CD patients. Serum IL1RA and GZMB levels were markedly increased in CD patients, suggesting that these markers can serve as biomarkers to identify gut inflammation. Further studies will be required to evaluate this possibility.

• Authors: Tae Jun Kim, Ja Seol Koo, Sung Jin Kim, Sung Noh Hong, You Sun Kim, Suk-Kyun Yang, Young-Ho Kim
• Journal: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (Biomarkers) Vol. 23 Issue 2 Pg. 161-166 (Mar 2018) ISSN: 1366-5804 [Electronic] England
• PMID: 28972805 (Publication Type: Journal Article)

Chemical References

• Biomarkers
• CXCL1 protein, human
• Chemokine CXCL1
• Interleukin 1 Receptor Antagonist Protein
• GZMB protein, human
• Granzymes

Topics

• Adult
• Biomarkers (blood, metabolism)
• Chemokine CXCL1 (genetics, metabolism)
• Crohn Disease (blood, diagnosis, genetics)
• Enzyme-Linked Immunosorbent Assay
• Female
• Gene Expression Profiling
• Granzymes (blood, genetics)
• Humans
• Inflammation (genetics, metabolism)
• Interleukin 1 Receptor Antagonist Protein (blood, genetics)
• Intestinal Mucosa (metabolism, pathology)
• Male
• Middle Aged
• Oligonucleotide Array Sequence Analysis (methods)
• ROC Curve
• Young Adult