Polyphenolic extract attenuates fatty acid-induced steatosis and oxidative stress in hepatic and endothelial cells.

Abstract

PURPOSE:

Phenolic compounds (PC) of virgin olive oil exert several biochemical and pharmacological beneficial effects. Some dietary PC seem to prevent/improve obesity and metabolic-related disorders such as non-alcoholic fatty liver disease (NAFLD). We investigated the possible effects of PC extracted from olive pomace (PEOP) and of the main single molecules present in the extract (tyrosol, apigenin, oleuropein, p-coumaric and caffeic acid) in protecting hepatocytes and endothelial cells against triglyceride accumulation and oxidative stress.

METHODS:

Rat hepatoma and human endothelial cells were exposed to a mixture of oleate/palmitate to mimic the condition of NAFLD and atherosclerosis, respectively. Then, cells were incubated for 24 h in the absence or in the presence of PC or PEOP. Different parameters were evaluated, such as lipid accumulation and oxidative stress-related markers.

RESULTS:

In hepatic cells, expression of peroxisome proliferator-activated receptors (PPARs) and of stearoyl-CoA desaturase 1 (SCD-1) were assessed as index of lipid metabolism. In endothelial cells, expression of intercellular adhesion molecule-1 (ICAM-1), activation of nuclear factor kappa-B (NF-kB), release of nitric oxide (NO), and wound-healing rate were assessed as index of inflammation.

CONCLUSION:

PEOP extract ameliorated hepatic lipid accumulation and lipid-dependent oxidative imbalance thus showing potential applications as therapeutic agent tuning down hepatosteatosis and atherosclerosis.

• Authors: Laura Vergani, Giulia Vecchione, Francesca Baldini, Elena Grasselli, Adriana Voci, Piero Portincasa, Pier Francesco Ferrari, Bahar Aliakbarian, Alessandro A Casazza, Patrizia Perego
• Journal: European journal of nutrition (Eur J Nutr) Vol. 57 Issue 5 Pg. 1793-1805 (Aug 2018) ISSN: 1436-6215 [Electronic] Germany
• PMID: 28526925 (Publication Type: Journal Article)

Chemical References

• Fatty Acids
• Polyphenols

Topics

• Animals
• Cell Line, Tumor
• Cells, Cultured
• Endothelial Cells (drug effects, metabolism)
• Fatty Acids (adverse effects, metabolism)
• Hepatocytes (drug effects, metabolism)
• Humans
• Lipid Metabolism
• Liver
• Non-alcoholic Fatty Liver Disease (drug therapy, metabolism)
• Oxidative Stress (drug effects)
• Polyphenols (pharmacology)
• Rats