Cardioprotective effects of monocyte locomotion inhibitory factor on myocardial ischemic injury by targeting vimentin.

Abstract

Monocyte locomotion inhibitory factor (MLIF), a heat-stable pentapeptide produced by Entamoeba histolytica, has anti-inflammatory function and protective effect on ischemic stroke. In this study, we evaluated the effect of MLIF on myocardial ischemia. Mice were subjected to ischemia/reperfusion by occlusion of the left anterior descending artery (LAD). After sacrifice, the serum concentrations of cardiac troponin I (cTnI), creatine kinase (CK), lactate dehydrogenase (LDH) as well as the heart infarct size were measured. HE and TUNEL staining were used to observe the pathological damage and the apoptotic cells. For in vitro study, the oxygen-glucose deprivation(OGD) model was established in H9c2 cells. MTT assay and flow cytometry assay were performed to evaluate cell viability and apoptosis. The expression of JNK and caspase 3 was assessed by western blot analysis. Pull-down assay was used to detect the specific binding protein of MLIF in myocardial cells. MLIF significantly reduced the infarct size, and the cTnI, CK and LDH levels, amelioratived pathological damage and reduced the apopotosis compared with the myocardial I/R model group. MLIF improved cell survival and inhibited apoptosis and necrosis by inhibiting the p-JNK and cleaved caspase3 expression. Furthermore, the binding protein of MLIF in myocardial cells was vimentin. Inhibition of vimentin expression by withaferin A or vimentin siRNA repressed the protective effects of MLIF in OGD-provoked H9c2 cells. Taken together, our results demonstrate that the cardioprotective effects of MLIF on myocardial ischemia injury are related to reductions in the inflammatory response and apoptosis by targeting vimentin.

• Authors: Shu Jiang, Yulan Liu, Jing Wang, Yue Zhang, Yaocheng Rui, Yuefan Zhang, Tiejun Li
• Journal: Life sciences (Life Sci) Vol. 167 Pg. 85-91 (Dec 15 2016) ISSN: 1879-0631 [Electronic] Netherlands
• PMID: 27773717 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier Inc. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Cardiotonic Agents
• Oligopeptides
• Troponin I
• Vimentin
• monocyte locomotion inhibitory factor
• L-Lactate Dehydrogenase
• Creatine Kinase

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, therapeutic use)
• Apoptosis (drug effects)
• Cardiotonic Agents (chemistry, therapeutic use)
• Cell Line
• Creatine Kinase (blood)
• Entamoeba histolytica (chemistry)
• Heart (drug effects)
• L-Lactate Dehydrogenase (blood)
• Male
• Mice, Inbred C57BL
• Myocardial Infarction (blood, drug therapy, metabolism, pathology)
• Myocardial Reperfusion Injury (blood, drug therapy, metabolism, pathology)
• Myocardium (metabolism, pathology)
• Myocytes, Cardiac (drug effects, metabolism, pathology)
• Oligopeptides (chemistry, therapeutic use)
• Troponin I (blood)
• Vimentin (metabolism)