Melatonin protects against myocardial hypertrophy induced by lipopolysaccharide.

Abstract

Melatonin is thought to have the ability of antiatherogenic, antioxidant, and vasodilatory. It is not only a promising protective in acute myocardial infarction but is also a useful tool in the treatment of pathological remodeling. However, its role in myocardial hypertrophy remains unclear. In this study, we investigated the protective effects of melatonin on myocardial hypertrophy induced by lipopolysaccharide (LPS) and to identify their precise mechanisms. The cultured myocardial cell was divided into six groups: control group, LPS group, LPS + ethanol (4%), LPS + melatonin (1.5 mg/ml) group, LPS + melatonin (3 mg/ml) group, and LPS + melatonin (6 mg/ml) group. The morphologic change of myocardial cell was observed by inverted phase contrast microscope. The protein level of myocardial cell was measured by Coomassie brilliant blue protein kit. The secretion level of tumor necrosis factor-α (TNF-α) was evaluated by enzyme-linked immunosorbent assay (ELISA). Ca(2+) transient in Fura-2/AM-loaded cells was measured by Till image system. The expression of Ca(2+)/calmodulin-dependent kinase II (CaMKII) and calcineurin (CaN) was measured by Western blot analysis. Our data demonstrated that LPS induced myocardial hypertrophy, promoted the secretion levels of TNF-α, and increased Ca(2+) transient level and the expression of CaMKII and CaN. Administration of melatonin 30 min prior to LPS stimulation dose-dependently attenuated myocardial hypertrophy. In conclusion, the results revealed that melatonin had the potential to protect against myocardial hypertrophy induced by LPS in vitro through downregulation of the TNF-α expression and retains the intracellular Ca(2+) homeostasis.

• Authors: Qi Lu, Xin Yi, Xiang Cheng, Xiaohui Sun, Xiangjun Yang
• Journal: In vitro cellular & developmental biology. Animal (In Vitro Cell Dev Biol Anim) Vol. 51 Issue 4 Pg. 353-60 (Apr 2015) ISSN: 1543-706X [Electronic] Germany
• PMID: 25475041 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cardiotonic Agents
• Lipopolysaccharides
• Proteins
• Tumor Necrosis Factor-alpha
• Calcium-Calmodulin-Dependent Protein Kinase Type 2
• Calcineurin
• Melatonin
• Calcium

Topics

• Animals
• Calcineurin (metabolism)
• Calcium (metabolism)
• Calcium-Calmodulin-Dependent Protein Kinase Type 2 (metabolism)
• Cardiomegaly (pathology)
• Cardiotonic Agents (pharmacology)
• Cell Size (drug effects)
• Cells, Cultured
• Lipopolysaccharides (toxicity)
• Melatonin (pharmacology)
• Myocytes, Cardiac (drug effects, metabolism, pathology)
• Proteins (metabolism)
• Rats, Sprague-Dawley
• Tumor Necrosis Factor-alpha (metabolism)