Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: MATERIALS AND METHODS: The powdered aerial parts of Pyrrosia petiolosa were used to extract various fractions with ethanol, petroleum ether, ethyl acetate, N-butanol and aqueous. Qualitative phytochemical screening was performed on the ethanol extract, petroleum ether fraction, ethyl acetate fraction, N-butanol fraction and aqueous fraction. The agar diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were employed to evaluate antibacterial activity of the ethanol extract and fractions. The in vitro cytotoxicity of ethanol extract and fractions was determined using MTT assay. The anti-inflammatory activity was analyzed using the mouse ear swelling induced by xylene. RESULTS: The phytochemical screening revealed the presence of anthraquinones, flavonoids, terpenoids, steroids, saponins, phenols and reducing sugars in the extract and fractions. Antibacterial results showed that petroleum ether fraction and N-butanol fraction inhibited all the tested microorganisms with the maximum inhibition zone of 15.25±0.35 mm. Ethyl acetate fraction also exhibited good antibacterial activity except Pseudomonas aeruginosa ATCC 27853, while extract and aqueous fraction inhibited 8 out of 13 (61.5%) of the tested microorganisms. The MIC values of ethanol extract and fractions ranged from 1.25 to 10.00 mg/mL and most of the MBC values were equal or twice as high as the corresponding MIC values. The in vitro cytotoxicity showed the ethanol extract and fractions exhibited non-toxic or low toxic activity against lung cancer cell lines A549 and mouse spleen cells. In anti-inflammatory experiment, ethanol extract at 5.0 and 10.0 mg/kg exhibited significant anti-inflammatory activity against the mouse ear swelling induced by xylene and the maximum inhibition rate reached as high as 67%. CONCLUSIONS: Pyrrosia petiolosa could be a potential candidate for future development of a novel antibacterial and anti-inflammatory agent.
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Authors | Dandan Cheng, Yingying Zhang, Demin Gao, Hongmeng Zhang |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 155
Issue 2
Pg. 1300-5
(Sep 11 2014)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 25077464
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Bacterial Agents
- Anti-Inflammatory Agents
- Plant Extracts
- Solvents
- Xylenes
- Ethanol
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Topics |
- Animals
- Anti-Bacterial Agents
(chemistry, isolation & purification, pharmacology, toxicity)
- Anti-Inflammatory Agents
(chemistry, isolation & purification, pharmacology, toxicity)
- Bacteria
(drug effects, growth & development)
- Cell Line, Tumor
- Disease Models, Animal
- Disk Diffusion Antimicrobial Tests
- Drug Stability
- Edema
(chemically induced, prevention & control)
- Ethanol
(chemistry)
- Humans
- Inflammation
(chemically induced, prevention & control)
- Phytotherapy
- Plant Components, Aerial
- Plant Extracts
(chemistry, isolation & purification, pharmacology, toxicity)
- Plants, Medicinal
- Polypodiaceae
(chemistry)
- Solvents
(chemistry)
- Xylenes
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