Abstract | OBJECTIVE: Black seed oil (BSO) is widely used as a traditional medicine for asthma and other inflammatory diseases. The aim of this study is to evaluate the effects of BSO on ovalbumin (OVA) induced acute lung remodelling in E3 inbred rats. METHOD: Rats were divided into three groups; Control, OVA and BSO. The rats were intraperitoneally sensitised and challenged intranasally with OVA and treated intraperitoneally with pure BSO for seven days. The collagen deposition and other pathological alteration were determined by Masson's trichrome, PAS and HE staining. Activity of arginase, ornithine decarboxylase (ODC) and proline level was determined by spectrophotometry, and polyamine by HPLC. The mRNA expression of arginase І, endothelin1 (Edn1), matrix metallopeptidase 3 (MMP3) and growth factors was determined by real time RT-PCR. RESULTS: CONCLUSION: Administration of BSO significantly reduced the level of allergen induced lung remodelling. The effect of BSO on lung remodelling is probably mediated by the inhibition of arginase pathways and the expression of Edn1, MMP3 and growth factors. Our findings suggest that BSO might have useful implications in the treatment and future research into allergen-induced lung remodelling.
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Authors | M B Raza Asim, Muhammad Shahzad, Xudong Yang, Qingzhu Sun, Fujun Zhang, Yan Han, Shemin Lu |
Journal | Swiss medical weekly
(Swiss Med Wkly)
Vol. 140
Pg. w13128
( 2010)
ISSN: 1424-3997 [Electronic] Switzerland |
PMID | 21136334
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plant Oils
- Ovalbumin
- caraway oil
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Topics |
- Animals
- Asthma
(chemically induced, complications)
- Female
- Lung Diseases
(etiology, prevention & control)
- Male
- Ovalbumin
(administration & dosage)
- Plant Oils
(therapeutic use)
- Rats
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