Abstract | AIM: METHODS: RV hypertrophy was induced in male Sprague-Dawley rats with monocrotaline (MCT; 60 mg kg(-1) ) and was confirmed by the presence of an increased RV weight and RV-to-[left ventricle (LV) and septum] ratio. RESULTS: in the RV of MCT-treated rats, a approximately 40% reduction in OTR mRNA and protein was observed compared with the RV of control rats. This reduction was associated with increased transcripts of ANP and BNP in both ventricles and a corresponding increase in NP receptor mRNA expression for receptors A, B and C. Protein expression of inducible NOS was increased in the RV, whereas endothelial NOS transcripts were increased only in the LV of MCT-treated rats. In the RV of MCT-treated rats, downregulation of OTR was also associated with increased mRNA expression of IL-1β and IL-6. CONCLUSION: our results show that downregulation of the OTR in the RV of MCT-treated rats is associated with increased expression of NP and their receptors as well as IL-1β and IL-6. This reduction in OTR in RV myocardium may have an impact on cardiac function in the MCT-induced model of PH.
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Authors | T L Broderick, Y Wang, J Gutkowska, D Wang, M Jankowski |
Journal | Acta physiologica (Oxford, England)
(Acta Physiol (Oxf))
Vol. 200
Issue 2
Pg. 147-58
(Oct 2010)
ISSN: 1748-1716 [Electronic] England |
PMID | 20377540
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Receptors, Oxytocin
- Oxytocin
- Monocrotaline
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Topics |
- Animals
- Blotting, Western
- Down-Regulation
- Gene Expression
(drug effects)
- Heart Ventricles
(drug effects, metabolism)
- Hypertension, Pulmonary
(chemically induced, metabolism)
- Male
- Monocrotaline
(toxicity)
- Oxytocin
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Receptors, Oxytocin
(biosynthesis, drug effects)
- Reverse Transcriptase Polymerase Chain Reaction
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