Abstract | OBJECTIVE: Age and excessive energy intake/ obesity are risk factors for cerebrovascular disease, but it is not known if and how these factors affect the extent of brain damage and outcome in ischemic stroke. We therefore determined the interactions of age and energy intake on the outcome of ischemic brain injury, and elucidated the underlying mechanisms. METHODS: We utilized a novel microchip-based immunoaffinity capillary electrophoresis technology to measure a panel of neurotrophic factors, cytokines, and cellular stress resistance proteins in brain tissue samples from young, middle-aged, and old mice that had been maintained on control or energy-restricted diets prior to middle cerebral artery occlusion and reperfusion. RESULTS: INTERPRETATION: Reduction in dietary energy intake differentially modulates neurotrophic and inflammatory pathways to protect neurons against ischemic injury, and these beneficial effects of IF are compromised during aging, resulting in increased brain damage and poorer functional outcome.
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Authors | Thiruma V Arumugam, Terry M Phillips, Aiwu Cheng, Christopher H Morrell, Mark P Mattson, Ruiqian Wan |
Journal | Annals of neurology
(Ann Neurol)
Vol. 67
Issue 1
Pg. 41-52
(Jan 2010)
ISSN: 1531-8249 [Electronic] United States |
PMID | 20186857
(Publication Type: Journal Article, Research Support, N.I.H., Intramural)
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Topics |
- Age Factors
- Aging
(metabolism, pathology)
- Animals
- Brain
(metabolism, pathology)
- Cell Death
- Cerebral Cortex
(metabolism, pathology)
- Corpus Striatum
(metabolism, pathology)
- Diet, Reducing
- Electrophoresis, Microchip
- Fasting
(metabolism)
- Infarction, Middle Cerebral Artery
(metabolism, mortality, pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Neuropsychological Tests
- Random Allocation
- Reperfusion Injury
(metabolism, mortality, pathology)
- Stroke
(metabolism, mortality, pathology)
- Time Factors
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