Abstract | BACKGROUND: AIM OF THE STUDY: To determine the presence of NKT cells in lesional AE skin in comparison with other eczematous disorders and to analyse their cytokine expression. METHODS: Immunofluorescence stainings were carried out using antibodies recognizing NKT cells, CD3+ and CD4+ cells, IFN-gamma and IL-4. RESULTS: Natural killer T cells have been detected in small numbers in the majority of AE specimens as well as in atopy patch test ( APT) reactions, allergic contact dermatitis (ACD) and irritant contact dermatitis (ICD). In AE, the proportion of NKT cells among CD3+ cells was approximately 5%. NKT cells expressed both IFN-gamma and IL-4 in AE, APT and ACD but predominantly IFN-gamma in ICD. CONCLUSION: Natural killer T cells are part of the inflammatory infiltrate of AE as well as APT, ACD and ICD, suggesting a pathogenic role of NKT cells in eczematous skin disorders. The pattern of IFN-gamma and IL-4 cytokine expression by NKT cells varied depending on the type of eczematous disease.
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Authors | D Simon, E Kozlowski, H Simon |
Journal | Allergy
(Allergy)
Vol. 64
Issue 11
Pg. 1681-4
(Nov 2009)
ISSN: 1398-9995 [Electronic] Denmark |
PMID | 19735490
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Interleukin-4
- Interferon-gamma
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Topics |
- Cytokines
(metabolism)
- Dermatitis, Allergic Contact
(immunology, pathology)
- Dermatitis, Atopic
(immunology, metabolism, pathology)
- Dermatitis, Irritant
(immunology, pathology)
- Humans
- Interferon-gamma
(metabolism)
- Interleukin-4
(metabolism)
- Natural Killer T-Cells
(cytology, immunology, metabolism)
- Skin
(immunology, metabolism, pathology)
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