Abstract | BACKGROUND AND AIMS: Imbalance between pro- and anti-inflammatory cytokines produced by intestinal T cells induces inflammatory bowel diseases (IBD). However, the importance of regulation of cytokine signalling in IBD has not been fully clarified. We have demonstrated that suppressor of cytokine signalling 1 (SOCS1) is expressed in inflamed tissues in an experimental colitis model. In the present study, we investigated the role of SOCS1 in colitis models to clarify the mechanism of IBD development. METHODS: Intestinal T cells in transgenic mice expressing high levels of SOCS1 in lymphocytes (SOCS1Tg mice) were characterised by flow cytometric analysis and cytokine production from intestinal T cells was determined by ELISA. 2,4,6-Trinitrobenzene sulphonic acid (TNBS) induced colitis was induced in SOCS1Tg mice and severity was compared with control littermates by measurement of survival rates. Intracellular signalling was assessed by western blotting analysis. RESULTS: CONCLUSIONS: Our data suggest that SOCS1 plays an important role in the regulation of colitis by controlling intestinal T cell activation mediated through CTLA-4 expression.
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Authors | K Inagaki-Ohara, A Sasaki, G Matsuzaki, T Ikeda, M Hotokezaka, K Chijiiwa, M Kubo, H Yoshida, Y Nawa, A Yoshimura |
Journal | Gut
(Gut)
Vol. 55
Issue 2
Pg. 212-9
(Feb 2006)
ISSN: 0017-5749 [Print] England |
PMID | 16120757
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD
- Antigens, Differentiation
- CTLA-4 Antigen
- Carrier Proteins
- Ctla4 protein, mouse
- Cytokines
- Repressor Proteins
- Socs1 protein, mouse
- Suppressor of Cytokine Signaling 1 Protein
- Suppressor of Cytokine Signaling Proteins
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Topics |
- Animals
- Antigens, CD
- Antigens, Differentiation
(immunology)
- CTLA-4 Antigen
- Carrier Proteins
(genetics, immunology, metabolism)
- Cells, Cultured
- Cytokines
(biosynthesis)
- Disease Models, Animal
- Down-Regulation
(immunology)
- Gene Expression Regulation
(immunology)
- Immunophenotyping
- Inflammatory Bowel Diseases
(immunology)
- Lymphocyte Activation
(immunology)
- Lymphocytes
(immunology)
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Repressor Proteins
(genetics, immunology, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Suppressor of Cytokine Signaling 1 Protein
- Suppressor of Cytokine Signaling Proteins
(genetics, immunology, metabolism)
- T-Lymphocyte Subsets
(immunology)
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