Glucose-induced enhanced anti-oxidant activity inhibits apoptosis in goose fatty liver.

Abstract

The development of mammalian nonalcoholic fatty liver disease is associated with oxidative stress, reduced mitochondrial function, and increased apoptosis in hepatocytes; however, the expressions of mitochondria-related genes are elevated in goose fatty liver, suggesting that there may be a unique protective mechanism in goose fatty liver. The aim of the study was to investigate this protective mechanism in terms of anti-oxidant capacity. Our data showed no substantial differences in the mRNA expression levels of the apoptosis-related genes including B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax), cysteinyl aspartate-specific proteinase-3 (Caspase-3), and cysteinyl aspartate-specific proteinase-9 (Caspase-9) in the livers of the control and overfeeding Lander geese groups. The protein expression levels of Caspase-3 and cleaved Caspase-9 were not markedly different between the groups. Compared with the control group, malondialdehyde content was significantly lower (P < 0.01), glutathione peroxidase (GSH-Px) activity, glutathione (GSH) content, and mitochondrial membrane potential levels were higher (P < 0.01) in the overfeeding group. The mRNA expression levels of the anti-oxidant genes superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPX1), and glutathione peroxidase 2 (GPX2) were increased in goose primary hepatocytes after 40 mM and 60 mM glucose treatment. Reactive oxygen species (ROS) levels were significantly reduced (P < 0.01), whereas the mitochondrial membrane potential was maintained at normal levels. The mRNA expression levels of the apoptosis-related genes Bcl-2, Bax, and Caspase-3 were not substantial. There were no significant differences in the expression levels of Caspase-3 and cleaved Caspase-9 proteins. In conclusion, glucose-induced enhanced anti-oxidant capacity may help protect the function of mitochondria and inhibit the occurrence of apoptosis in goose fatty liver.

• Authors: Qingyun Sun, Erpeng Dai, Meng Chen, Jinqi Zhang, Ji'an Mu, Long Liu, Tuoyu Geng, Daoqing Gong, Yanjun Zhang, Minmeng Zhao
• Journal: Journal of animal science (J Anim Sci) Vol. 101 (Jan 03 2023) ISSN: 1525-3163 [Electronic] United States
• PMID: 36808418 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Antioxidants
• Glucose
• Caspase 3
• Caspase 9
• bcl-2-Associated X Protein
• Aspartic Acid
• Proto-Oncogene Proteins c-bcl-2
• Glutathione
• Glutathione Peroxidase
• RNA, Messenger

Topics

• Animals
• Antioxidants (metabolism)
• Geese (genetics, metabolism)
• Glucose (metabolism)
• Caspase 3 (genetics, metabolism)
• Caspase 9 (metabolism)
• bcl-2-Associated X Protein (genetics, metabolism)
• Aspartic Acid (metabolism)
• Fatty Liver (veterinary)
• Liver (metabolism)
• Apoptosis
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Oxidative Stress
• Glutathione (metabolism)
• Glutathione Peroxidase (metabolism)
• RNA, Messenger (metabolism)
• Mammals (genetics)