Abstract | AIMS: MAIN METHODS: Wistar male rats (n = 50) were categorized equally into 5 groups (baseline, sham+saline, BDL + saline, sham+NTX (10 mg/kg of body weight (BW)), and BDL + NTX (10 mg/kg of BW)). At the end of the experiment, H&E staining was used to assess necrosis and lobular damage of hepatic tissue. The gene expression of THBS-1 and NADPH oxidase 1 (NOX-1) was measured by real time-PCR and VEGF-A, Nrf-2, Keap-1, and TGF-β1 protein levels were assessed by western blot. The antioxidant enzymes activity, total oxidant status (TOS) and MDA level were measured by commercial kits. KEY FINDINGS: Hepatic necrosis and lobular damage increased substantially and NTX reduced them markedly in the BDL group. Gene expression of hepatic THBS-1 and NOX-1, TOS and MDA levels increased markedly in the BDL + saline group, and Nrf-2 and VEGF-A values decreased significantly in the BDL + NTX group. NTX recovered THBS-1, NOX-1 and Nrf-2 in the BDL + NTX group, substantially (p-value ≤ 0.05). SIGNIFICANCE:
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Authors | Seyed Reza Hosseini-Fard, Ahmad Reza Dehpour, Solaleh Emamgholipour, Abolfazl Golestani |
Journal | Life sciences
(Life Sci)
Vol. 300
Pg. 120576
(Jul 01 2022)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 35487305
(Publication Type: Journal Article)
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Copyright | Copyright © 2022. Published by Elsevier Inc. |
Chemical References |
- Antioxidants
- Thrombospondin 1
- Transforming Growth Factor beta1
- Vascular Endothelial Growth Factor A
- Naltrexone
- NADPH Oxidase 1
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Topics |
- Animals
- Antioxidants
(metabolism, pharmacology)
- Bile Ducts
(metabolism)
- Ligation
- Liver
(metabolism)
- Liver Cirrhosis
(pathology)
- Male
- NADPH Oxidase 1
(metabolism)
- Naltrexone
(metabolism, pharmacology)
- Necrosis
(metabolism)
- Rats
- Rats, Wistar
- Thrombospondin 1
(metabolism)
- Transforming Growth Factor beta1
(metabolism)
- Vascular Endothelial Growth Factor A
(metabolism)
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