Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis.

Abstract

Florfenicol (FFC) is a commonly used antibiotic in animal husbandry, which is easy to cause organs damage in a variety of animals. It has been proved to have nephrotoxicity and affect the yield and quality of meat products. Salvia miltiorrhiza polysaccharides (SMPs) have been proved to have the pharmacological effects of regulating immunity and protecting the liver of animals, and its alleviative effect on renal injury is unclear. In order to investigate the alleviating effect of SMPs on drug nephrotoxicity and determine its potential molecular mechanism, we took chicks as the research object, FFC as the induced drug, and established the model by adding SMPs in drinking water. The chicks were randomly divided into control group, FFC model group (0.15 g/L FFC), FFC + low, medium and high dose of SMPs groups (0.15 g/L FFC + 1.25, 2.5, 5 g/L SMPs) and SMPs group (5 g/L SMPs). The results showed that, SMPs increased the average weight gain and renal index of chicks, alleviated the pathological changes of renal structure induced by FFC, decreased the contents of uric acid, blood urea nitrogen and creatinine in serum and malondialdehyde in renal tissue, increased the levels of glutathione, superoxide dismutase and catalase in renal tissue, up-regulated the relative expression levels of nuclear factor erythroid 2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase-1 (NQO-1) mRNA and protein, and down-regulated the relative expression levels of p53, Caspase-3 and Caspase-6 mRNA and protein and the apoptosis rate of renal histiocytes. It is concluded that SMPs could significantly alleviate the renal injury induced by FFC, and its mechanism may be related to improving renal antioxidant capacity and inhibiting abnormal apoptosis of renal histiocytes.

• Authors: Xiao Wang, Wei Liu, Guozhong Jin, Zhanjun Wu, Di Zhang, Yongzhan Bao, Wanyu Shi
• Journal: Ecotoxicology and environmental safety (Ecotoxicol Environ Saf) Vol. 233 Pg. 113339 (Mar 15 2022) ISSN: 1090-2414 [Electronic] Netherlands
• PMID: 35219255 (Publication Type: Journal Article, Randomized Controlled Trial, Veterinary)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• NF-E2-Related Factor 2
• Polysaccharides
• florfenicol
• Thiamphenicol

Topics

• Animals
• Apoptosis
• Kidney
• NF-E2-Related Factor 2 (genetics, metabolism)
• Oxidative Stress
• Polysaccharides (metabolism, pharmacology)
• Salvia miltiorrhiza (chemistry)
• Thiamphenicol (analogs & derivatives)