Abstract | ABSTRACT: Although inflammation plays an important role in myocardial ischemia/ reperfusion injury (MI/RI), an anti-inflammatory treatment with a single target has little clinical efficacy because of the multifactorial disorders involved in MI/RI. MicroRNAs (miR-24) can achieve multitarget regulation in several diseases, suggesting that this factor may have ideal effects on alleviation of MI/RI. In the present study, bioinformatics method was used to screen potential therapeutic targets of miR-24 associated with MI/RI. Three days before ischemia/reperfusion surgery, rats in the ischemia/reperfusion, miR-24, and adenovirus-negative control groups were injected with saline, miR-24, and adenovirus-negative control (0.1 mL of 5 × 109 PFU/mL), respectively. Myocardial enzymes, myocardial infarct size, cardiac function, and the possible molecular mechanism were subsequently analyzed. In contrast to the level of S100A8, the level of miR-24 in myocardial tissue was significantly reduced after 30 minutes of ischemia followed by reperfusion for 2 hours. Overexpression of miR-24 reduced the myocardial infarction area and improved the heart function of rats 3 days after MI/RI. Moreover, miR-24 inhibited infiltration of inflammatory cells in the peri- infarction area and decreased creatine kinase myocardial band and lactate dehydrogenase release. Interestingly, miR-24 upregulation reduced S100A8 expression, followed by inhibition of toll-like receptor 4/MyD-88/nuclear factor-k-gene binding signaling activation. In conclusion, miR-24 can alleviate MI/RI via inactivation of the S100A8/ toll-like receptor 4/MyD-88/nuclear factor-k-gene binding signaling pathway.
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Authors | Jian Yang, Zujin Xiang, Jing Zhang, Jun Yang, Yuhong Zhai, Zhixing Fan, Huibo Wang, Jingyi Wu, Yifan Huang, Mengting Xiong, Cong Ma |
Journal | Journal of cardiovascular pharmacology
(J Cardiovasc Pharmacol)
Vol. 78
Issue 6
Pg. 847-857
(12 01 2021)
ISSN: 1533-4023 [Electronic] United States |
PMID | 34581696
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved. |
Chemical References |
- Calgranulin A
- MIRN24 microRNA, rat
- MicroRNAs
- Myd88 protein, rat
- Myeloid Differentiation Factor 88
- NF-kappa B
- Tlr4 protein, rat
- Toll-Like Receptor 4
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Topics |
- Animals
- Calgranulin A
(genetics, metabolism)
- Disease Models, Animal
- Male
- MicroRNAs
(genetics, metabolism)
- Myeloid Differentiation Factor 88
(metabolism)
- Myocardial Infarction
(genetics, metabolism, pathology, prevention & control)
- Myocardial Reperfusion Injury
(genetics, metabolism, pathology, prevention & control)
- Myocardium
(metabolism, pathology)
- NF-kappa B
(metabolism)
- Rats, Sprague-Dawley
- Signal Transduction
- Toll-Like Receptor 4
(metabolism)
- Ventricular Function, Left
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