Abstract | AIMS: MAIN METHODS: Wistar rats were randomly divided into six groups (n = 8 per group): sham, ovariectomized group (OVX), OVX + diabetes (OVX.D), OVX.D+ genistein (1 mg/kg, eight weeks; daily SC), OVX.D + exercise (eight weeks), and OVX.D+ genistein+exercise (eight weeks). At the end of 8 weeks, the retina was removed under anesthesia. The assessed effects of treatment were by measuring MiR-146a and miR-132 expression via RT-PCR, the protein levels of ERK, MMP-2, VEGF, and NF-κB via western blotting, inflammation, and oxidative stress markers levels via the Eliza. KEY FINDINGS: The results showed miR-132, miR-146b, and MMP-2, NF-κB, ERK, VEGF, TNF-α, IL-1β proteins, and MDA factor in the OVX.D group were increased, but glutathione (GSH) was decreased in comparison with the sham and OVX groups. Both exercise and genistein treatment has reversed the disorder caused by diabetes. However, the combination of exercise and genistein was more effective than each treatment alone. SIGNIFICANCE:
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Authors | Reihaneh Sadeghian, Siamak Shahidi, Alireza Komaki, Parisa Habibi, Nasser Ahmadiasl, Hadi Yousefi, Faeze Daghigh |
Journal | Life sciences
(Life Sci)
Vol. 284
Pg. 119931
(Nov 01 2021)
ISSN: 1879-0631 [Electronic] Netherlands |
PMID | 34480934
(Publication Type: Journal Article)
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Copyright | Copyright © 2021. Published by Elsevier Inc. |
Chemical References |
- Biomarkers
- Inflammation Mediators
- MicroRNAs
- NF-kappa B
- Vascular Endothelial Growth Factor A
- Malondialdehyde
- Genistein
- Extracellular Signal-Regulated MAP Kinases
- Matrix Metalloproteinase 2
- Glutathione
- Glucose
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Topics |
- Animals
- Biomarkers
(metabolism)
- Diabetes Mellitus, Experimental
(metabolism, pathology)
- Extracellular Signal-Regulated MAP Kinases
(metabolism)
- Female
- Genistein
(administration & dosage, pharmacology)
- Glucose
(metabolism)
- Glutathione
(metabolism)
- Homeostasis
(drug effects)
- Inflammation
(pathology)
- Inflammation Mediators
(metabolism)
- Malondialdehyde
(metabolism)
- Matrix Metalloproteinase 2
(metabolism)
- MicroRNAs
(genetics, metabolism)
- NF-kappa B
(metabolism)
- Ovariectomy
- Oxidative Stress
(drug effects)
- Phosphorylation
(drug effects)
- Rats, Wistar
- Retina
(drug effects, pathology)
- Swimming
(physiology)
- Vascular Endothelial Growth Factor A
(metabolism)
- Rats
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