Recent studies have implicated
mitochondrial dysfunction as a trigger of
inflammatory bowel diseases, including
Crohn's disease (CD) and
ulcerative colitis (UC). We have investigated the role of the mitochondria gate-keeper
protein, the
voltage-dependent-anion channel 1 (VDAC1), in gastrointestinal
inflammation and tested the effects of the newly developed VDAC1-interacting molecules, VBIT-4 and VBIT-12, on UC induced by
dextran sulfate sodium (DSS) or trinitrobenzene sulphonic
acid (TNBS) in mice. VDAC1, which controls metabolism,
lipids transport, apoptosis, and
inflammasome activation, is overexpressed in the colon of CD and UC patients and DSS-treated mice. VBIT-12 treatment of cultured colon cells inhibited the DSS-induced VDAC1 overexpression, oligomerization, and apoptosis. In the DSS-treated mice, VBIT-12 suppressed
weight loss,
diarrhea, rectal
bleeding, pro-inflammatory
cytokine production, crypt and epithelial cell damage, and focal
inflammation. VBIT-12 also inhibited the infiltration of inflammatory cells, apoptosis,
mtDNA release, and activation of caspase-1 and NRLP3
inflammasome to reduce the inflammatory response. The levels of the
ATP-gated P2X7-Ca2+/K+ channel and ER-IP3R-Ca2+ channel, and of the mitochondrial anti-
viral protein (MAVS), mediating NLRP3
inflammasome assembly and activation, were highly increased in DSS-treated mice, but not when VBIT-12 treated. We conclude that UC may be promoted by VDAC1-overexpression and may therefore be amenable to treatment with novel VDAC1-interacting molecules. This VDAC1-based strategy exploits a completely new target for UC treatment and opens a new avenue for treating other inflammatory/
autoimmune diseases.