Cell Division Cycle 2 Protects Neonatal Rats Against Hyperoxia-Induced Bronchopulmonary Dysplasia.

Abstract

PURPOSE:

Hyperoxia-induced bronchopulmonary dysplasia (BPD) is a lung disease in preterm infants. We aimed to explore the role of cell division cycle 2 (CDC2) on histopathologic changes of lung tissues, as well as the viability, apoptosis, and inflammation of lung cells in rats with hyperoxia-induced BPD.

MATERIALS AND METHODS:

Hyperoxia-induced BPD in neonatal rats and hyperoxia-induced A549 cells were constructed. The mRNA expression of CDC2 was detected by qRT-PCR. The fibrosis score of lung tissues was evaluated by hematoxylin-eosin staining. The viability and apoptosis of A549 cells were detected by cell counting kit-8 assay and flow cytometry. The protein expressions of bcl-2, bax, and caspase-3 were measured by western blot. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and IL-1β in A549 cells were detected by enzyme-linked immunosorbent assay. The pcDNA3.1-CDC2 was injected into rats to determine the role of CDC2 in hyperoxia-induced BPD in vivo.

RESULTS:

The expression of CDC2 was decreased in lung tissues of neonatal rats with hyperoxia-induced BPD and hyperoxia-induced A549 cells. The fibrosis score was increased in the lung tissues of neonatal rats with hyperoxia-induced BPD. Overexpression of CDC2 increased the viability and protein expression of bcl-2; and inhibited the apoptosis, inflammation, and protein expression of bax and caspase-3 in hyperoxia-induced A549 cells. Up-regulation of CDC2 alleviated the histopathologic changes in lung tissues of neonatal rats with hyperoxia-induced BPD.

CONCLUSION:

Overexpression of CDC2 promoted the viability and inhibited the apoptosis and inflammation of hyperoxia-induced cells, and alleviated the histopathologic changes of lung tissues in neonatal rats with hyperoxia-induced BPD.

• Authors: Zhongying Li, Yanhong Chen, Wenrong Li, Fan Yan
• Journal: Yonsei medical journal (Yonsei Med J) Vol. 61 Issue 8 Pg. 679-688 (Aug 2020) ISSN: 1976-2437 [Electronic] Korea (South)
• PMID: 32734731 (Publication Type: Journal Article)
• Copyright: © Copyright: Yonsei University College of Medicine 2020.

Chemical References

• Cytokines
• Inflammation Mediators
• CDC2 Protein Kinase

Topics

• A549 Cells
• Animals
• Animals, Newborn
• Apoptosis
• Bronchopulmonary Dysplasia (etiology, prevention & control)
• CDC2 Protein Kinase (metabolism)
• Cell Cycle
• Cell Survival
• Cytokines (metabolism)
• Disease Models, Animal
• Humans
• Hyperoxia (complications, pathology)
• Inflammation Mediators (metabolism)
• Lung (pathology)
• Rats, Wistar