Abstract | BACKGROUND: Acute respiratory viral infections are a major cause of respiratory morbidity and mortality, especially in patients with preexisting lung diseases such as asthma. Toll-like receptors are critical in the early detection of viruses and in activating innate immunity in the respiratory mucosa, but there is no reliable and convenient method by which respiratory mucosal innate immune responses can be measured. OBJECTIVE: We sought to assess in vivo immune responses to an innate stimulus and compare responsiveness between healthy volunteers and volunteers with allergy. METHODS: RESULTS: R848 was well tolerated and significantly induced IFN-α2a, IFN-γ, proinflammatory cytokines (TNF-α, IL-2, IL-12p70), and chemokines (CXCL10, C-C motif chemokine ligand [CCL]2, CCL3, CCL4, and CCL13) in nasal mucosal fluid, without causing systemic immune activation. Participants with allergic rhinitis or allergic rhinitis with asthma had increased IFN-α2a, CCL3, and CCL13 responses relative to healthy participants; those with asthma had increased induction of IFN-stimulated genes DExD/H-box helicase 58, MX dynamin-like GTPase 1, and IFN-induced protein with tetratricopeptide repeats 3. CONCLUSIONS: Responses to nasal delivery of R848 enables simple assessment of mucosal innate responsiveness, revealing that patients with allergic disorders have an increased nasal mucosal IFN and chemokine response to the viral RNA analogue R848. This highlights that dysregulated innate immune responses of the nasal mucosa in allergic individuals may be important in determining the outcome of viral exposure.
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Authors | Akhilesh Jha, Ryan S Thwaites, Tanushree Tunstall, Onn Min Kon, Robin J Shattock, Trevor T Hansel, Peter J M Openshaw |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 147
Issue 2
Pg. 694-703.e12
(02 2021)
ISSN: 1097-6825 [Electronic] United States |
PMID | 32717253
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- CCL13 protein, human
- Imidazoles
- Monocyte Chemoattractant Proteins
- TLR7 protein, human
- TLR8 protein, human
- Toll-Like Receptor 7
- Toll-Like Receptor 8
- Interferons
- resiquimod
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Topics |
- Adult
- Asthma
(immunology)
- Female
- Humans
- Imidazoles
(pharmacology)
- Immunity, Innate
(drug effects, immunology)
- Interferons
(immunology)
- Male
- Monocyte Chemoattractant Proteins
(immunology)
- Nasal Mucosa
(drug effects, immunology)
- Rhinitis, Allergic
(immunology)
- Toll-Like Receptor 7
(agonists)
- Toll-Like Receptor 8
(agonists)
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