Abstract |
Acute gouty inflammation could be triggered by phagocytosis of monosodium urate (MSU) by immune cells. This study investigated the protective effect and underlying mechanism of docosahexaenoic acid (DHA) on MSU-induced inflammation in vitro and in vivo. Results showed that DHA effectively inhibited MSU-induced expression and secretion of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in THP-1 cells. Intracellular reactive oxygen species (ROS) production triggered by MSU was alleviated by DHA treatment. Furthermore, DHA promoted the nuclear translocation of nuclear factor E2-related factor 2 (Nrf2), wherein Nrf2 further mediated the expression of multiple antioxidant enzymes such as, heme oxygenase-1 (HO-1), NAD(P)H: quinone oxidoreductase-1 (NQO1) and catalase, which are closely related with redox homeostasis. DHA treatment also restored MSU-induced impairment of mitochondrial transmembrane potential. In addition, oral administration of DHA-rich microalgal oil to C57BL/6 mice effectively reduced the infiltration of neutrophils, and decreased the expression and secretion of inflammatory cytokines. Altogether, our results suggest that DHA or DHA-rich microalgal oil may be a promising natural agent for the prevention of MSU-induced inflammation and potentially acute gout at least partly by attenuating oxidative stress.
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Authors | Yue Zhang, Lu Liu, Dongzhe Sun, Yongjing He, Yue Jiang, Ka-Wing Cheng, Feng Chen |
Journal | Food & function
(Food Funct)
Vol. 10
Issue 7
Pg. 4010-4021
(Jul 17 2019)
ISSN: 2042-650X [Electronic] England |
PMID | 31214670
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Cytokines
- IL1B protein, human
- Interleukin-1beta
- NF-E2-Related Factor 2
- Protective Agents
- Reactive Oxygen Species
- Tumor Necrosis Factor-alpha
- Docosahexaenoic Acids
- Uric Acid
- Catalase
- Heme Oxygenase-1
- NAD(P)H Dehydrogenase (Quinone)
- NQO1 protein, human
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Topics |
- Animals
- Antioxidants
(pharmacology)
- Catalase
(metabolism)
- Cell Survival
(drug effects)
- Cytokines
(metabolism)
- Docosahexaenoic Acids
(pharmacology, therapeutic use)
- Gout
- Heme Oxygenase-1
(metabolism)
- Homeostasis
(drug effects)
- Humans
- Inflammation
(chemically induced, drug therapy, immunology)
- Interleukin-1beta
(metabolism)
- Male
- Membrane Potential, Mitochondrial
- Mice
- Mice, Inbred C57BL
- NAD(P)H Dehydrogenase (Quinone)
(metabolism)
- NF-E2-Related Factor 2
(metabolism)
- Neutrophils
(drug effects)
- Oxidative Stress
(drug effects)
- Protective Agents
(pharmacology)
- Reactive Oxygen Species
(metabolism)
- THP-1 Cells
- Tumor Necrosis Factor-alpha
(metabolism)
- Uric Acid
(adverse effects)
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