Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: AIM OF THE STUDY: The goal of this study was to clarify the effects of RRFs on triglyceride metabolism and its related mechanisms. MATERIALS AND METHODS: RRFs were obtained by ethanol extraction from Rosa rugosa Thunb.. Transgenic mice expressing human Apolipoprotein C3 (ApoC3) were used as a mouse model of hypertriglyceridemia. Fenofibrate (FNB), a PPARα agonist, was used as a positive control drug of decreasing high triglyceride. FNB (100 mg/kg) or RRFs (300 mg/kg) were given to the mice by gavage daily. Two weeks later, the changes of plasma lipid levels in the mice were measured by commercial kits, the clearance of triglyceride was evaluated by oral fat load test, and expression of the genes related to lipid β-oxidation and synthesis was detected in the mice livers by real time PCR. RESULTS: RRFs, as well as FNB, were found to significantly reduce plasma triglyceride (TG) levels in ApoC3 transgenic mice after administration of the drug for two weeks. Plasma lipid clearance rate was increased and lipid content in the mice livers was reduced after administration of RRF. Treatment with RRFs up-regulated mRNA expression of PPARα and its downstream gene of ACOX, while down-regulated mRNA expression of the genes related to fatty acid synthesis (FASN, SREBP-1c, and ACC1). The expression of LPL was raised, while the expression of ApoC3 was decreased, and Foxo1 was inhibited by RRFs in the mice livers. CONCLUSION: RRFs can reduce plasma TG levels by repressing the expression of ApoC3 and inducing the expression of LPL in liver. RRFs could also reduce triglyceride in hepatocytes through increasing β-oxidation and decreasing synthesis of the lipids. These findings show the potency of further clinical application of RRFs as a hypolipidemic drug for treatment of cardiovascular diseases.
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Authors | Asiya Baiyisaiti, Yuhui Wang, Xuehui Zhang, Wen Chen, Rong Qi |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 240
Pg. 111952
(Aug 10 2019)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 31100436
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 Elsevier B.V. All rights reserved. |
Chemical References |
- Apolipoprotein C-III
- Flavonoids
- Hypolipidemic Agents
- PPAR alpha
- Triglycerides
- Cholesterol
- Lipoprotein Lipase
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Topics |
- Animals
- Apolipoprotein C-III
(genetics)
- Cholesterol
(metabolism)
- Flavonoids
(pharmacology, therapeutic use)
- Hypertriglyceridemia
(drug therapy, metabolism)
- Hypolipidemic Agents
(pharmacology, therapeutic use)
- Lipid Metabolism
(drug effects)
- Lipoprotein Lipase
(genetics)
- Liver
(drug effects, metabolism)
- Male
- Mice
- Mice, Transgenic
- PPAR alpha
(agonists)
- Rosa
- Triglycerides
(metabolism)
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