Abstract | BACKGROUND: METHODS: RESULTS: Levels of U-C3M were significantly elevated 7 days after ischaemia reperfusion in the NTxIRI. BiIRI animals showed higher levels of U-C3M after 7 and 14 days of reperfusion but not at 21 days. P-C3M did not change in any of the models. There was a significant correlation between U-C3M and mRNA levels of fibronectin, COL I alpha 1 chain (COL Ia1) and neutrophil gelatinase-associated lipocalin (NGAL), and protein levels of alpha smooth muscle actin (αSMA), fibronectin and COL III in NTxIRI but not in NTx animals. Levels of U-C3M increased significantly in the BiIRI animals subsequent to reperfusion, and mirrored the histological alterations. Furthermore, U-C3M was associated with the extent of fibrosis, and remained elevated even after plasma creatinine levels decreased. CONCLUSIONS: These results demonstrate that degradation of COL III increases after ischaemia reperfusion injury, and that U-C3M may be a non-invasive marker of tissue repair and fibrosis in the ischaemic kidney.
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Authors | Daniel Guldager Kring Rasmussen, Per Mose Nielsen, Özlem Yashar Kasab-Oglo, Signe Holm Nielsen, Casper Kierulf-Lassen, Morten Asser Karsdal, Federica Genovese, Rikke Nørregaard |
Journal | Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
(Nephrol Dial Transplant)
Vol. 34
Issue 8
Pg. 1301-1309
(08 01 2019)
ISSN: 1460-2385 [Electronic] England |
PMID | 30535162
(Publication Type: Journal Article)
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Copyright | © The Author(s) 2018. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. |
Chemical References |
- Biomarkers
- Collagen Type III
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Topics |
- Animals
- Biomarkers
(blood, urine)
- Collagen Type III
(metabolism)
- Fibrosis
(diagnosis, etiology, metabolism)
- Kidney Function Tests
- Male
- Nephrectomy
(adverse effects)
- Rats
- Rats, Wistar
- Reperfusion Injury
(diagnosis, etiology, metabolism)
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