Tetrahydropalmatine Prevents High-Fat Diet-Induced Hyperlipidemia in Golden Hamsters (Mesocricetus Auratus).

Abstract

BACKGROUND Hyperlipidemia is a major cause of atherosclerotic cardiovascular disease. Tetrahydropalmatine (THP) can exhibit hepatoprotective, anti-arrhythmic, and anti-inflammatory activities. The mechanism of THP on the hyperlipidemia remains unknown; therefore, the present study explored the role of THP in hyperlipidemia. MATERIAL AND METHODS We established an animal model of hyperlipidemia by high-fat diet (HFD) feeding. Blood samples were obtained for determination of serum cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c), high-density lipoprotein cholesterol (HDL-c), pro-inflammatory cytokines, and CYP7A1 expression. Histology was performed and inflammation was detected in the liver using hematoxylin-eosin (HE) staining and enzyme-linked immunosorbent assay (ELISA), respectively. The mRNA and protein levels of TLR4 and TRAF-6 were determined by quantitative real-time PCR (qPCR) and Western blot, respectively. RESULTS THP suppressed hepatic lipid accumulation and reduced serum levels of TC, TG, LDL-c, and HDL-c in HFD-fed golden hamsters. THP increased cholesterol 7 a-hydroxylase (CYP7A1) expression and prevented inflammation by the limited reduction in interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) expressions in serum and liver. THP slightly increased the ratio of the body/liver weight. THP inhibited the mRNA and protein levels of Toll-like receptor 4 (TLR4) and TNF-receptor associated factor-6 (TRAF-6). CONCLUSIONS These results suggest that THP attenuates hyperlipidemia by multiple effects, including hepatoprotective and anti-inflammatory effects. Moreover, THP also suppressed the expressions of TLR4 and TRAF-6 in golden hamsters.

• Authors: Caihua Sun, Zhiyun Chen, Hui Wang, Ke Ding
• Journal: Medical science monitor : international medical journal of experimental and clinical research (Med Sci Monit) Vol. 24 Pg. 6564-6572 (Sep 18 2018) ISSN: 1643-3750 [Electronic] United States
• PMID: 30226834 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Berberine Alkaloids
• Cholesterol, HDL
• Cholesterol, LDL
• Cytokines
• Triglycerides
• tetrahydropalmatine
• Cholesterol
• Cholesterol 7-alpha-Hydroxylase

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Berberine Alkaloids (pharmacology)
• Cholesterol (blood)
• Cholesterol 7-alpha-Hydroxylase (blood)
• Cholesterol, HDL (blood)
• Cholesterol, LDL (blood)
• Cricetinae (metabolism)
• Cytokines (blood)
• Diet, High-Fat (adverse effects)
• Disease Models, Animal
• Hyperlipidemias (drug therapy)
• Liver (metabolism)
• Male
• Mesocricetus (metabolism)
• Triglycerides (blood)