Pinacidil-postconditioning is equivalent to ischemic postconditioning in defeating cardiac ischemia-reperfusion injury in rat.

Abstract

Ischemic postconditioning (IPO) had been reported as a promising method against myocardial ischemia-reperfusion (I/R) injury, but IPO was later proved with poor clinical benefit. In this study, we compared the protective effects of pinacidil-postconditioning (PPO) and IPO against myocardial I/R injury. Langendorff rat hearts were randomly assigned to one of the following groups (n=8 each): Control group, I/R group (40min ischemia and 60min reperfusion), IPO group (6 successive cycles of 10s reperfusion per 10s occlusion before fully reperfusion), PPO group (perfused with 50μM pinacidil for 5min before reperfusion). Heart performance, infarct size and mitochondrial respiratory function were evaluated, and target genes/proteins of well-known Nuclear Factor-E2 Related Factor 2 (Nrf2) were assessed. Both IPO and PPO preserved heart function and myocardial ultrastructure at the end of reperfusion (all P<0.05 vs. I/R). The expression of Nrf2, NADH-quinone oxidoreductase-1 (NQO1), heme oxygenase 1 (HO-1) and superoxide dismutase 1 (SOD1) were similarly increased after IPO and PPO treatment (all P<0.05 vs. I/R). PPO exerted solid effect in defeating cardiac ischemia-reperfusion injury in rat.

• Authors: Yi Hui Yang, Yu Zhang, Wei Chen, Ying Wang, Song Cao, Tian Yu, Haiying Wang
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 780 Pg. 26-32 (Jun 05 2016) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 26997370 (Publication Type: Journal Article)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• NF-E2-Related Factor 2
• Nfe2l2 protein, rat
• Pinacidil

Topics

• Animals
• Cell Respiration (drug effects)
• Coronary Vessels (drug effects, physiopathology)
• Hemodynamics (drug effects)
• Ischemic Postconditioning (methods)
• Male
• Mitochondria (drug effects, metabolism)
• Myocardial Reperfusion Injury (genetics, metabolism, pathology, physiopathology)
• Myocardium (metabolism, pathology)
• NF-E2-Related Factor 2 (metabolism)
• Pinacidil (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Up-Regulation (drug effects)