Effects of Kaurenoic Acid and Arginine on Random Skin Flap Oxidative Stress, Inflammation, and Cytokines in Rats.

Abstract

BACKGROUND:

Kaurenoic acid (KA), a diterpene extracted from copaíba oil-resin, is known to have potent antioxidant and anti-inflammatory properties. L-Arginine (LA) is an amino acid and a nitrogenous precursor for the synthesis of nitric oxide (NO). NO paper in wound healing has already been well documented. The aim of this study was to investigate the protective effects of LA and KA against ischemia reperfusion injury in a randomized skin flap model in rats.

METHODS:

A modified McFarlane flap model measuring 2.5 wide × 8 cm long was established in 36 anesthetized rats and evaluated within 3 groups: group control, group L-arginine, and group kaurenoic acid. Each group was subdivided into two subgroups (T1 and T2, n = 6 each). Samples were collected 24 h (T1)/48 h (T2) postoperatively for oxidative stress (glutathione), as non-protein thiols, malondialdehyde (MDA), NO2, inflammation [myeloperoxidase (MPO)], and cytokines TNF-α and IL-1β assays.

RESULTS:

KA promoted a significant decrease of TNF-α and IL-1 expression and MPO activity at T1/T2 time points. NSGH levels increased significantly in KA-treated rats, while MDA levels decreased significantly in the same rats. Arginine promoted a significant decrease in MDA levels at the T1 time point and a significant increase in non-protein thiols concentrations at T1/T2 time points. NO2 concentration also decreased at the T1 time point.

CONCLUSIONS:

KA may attenuate the oxidative stress and the inflammation, thereby reducing tissue damage induced by ischemia/reperfusion in rats subjected to dorsal skin flaps.

NO LEVEL ASSIGNED:

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• Authors: Joaquim José de Lima Silva, Débora Gramosa Pompeu, Naiara Coelho Ximenes, Antoniella Souza Gomes Duarte, Nilce Viana Gramosa, Krishnamurti de Morais Carvalho, Gerly Anne de Castro Brito, Sergio Botelho Guimarães
• Journal: Aesthetic plastic surgery (Aesthetic Plast Surg) Vol. 39 Issue 6 Pg. 971-7 (Dec 2015) ISSN: 1432-5241 [Electronic] United States
• PMID: 26408387 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Diterpenes
• kaurenoic acid
• Arginine

Topics

• Animals
• Arginine (pharmacology, therapeutic use)
• Cytokines (drug effects, physiology)
• Diterpenes (pharmacology, therapeutic use)
• Inflammation (prevention & control)
• Male
• Oxidative Stress (drug effects)
• Random Allocation
• Rats
• Rats, Wistar
• Reperfusion Injury (prevention & control)
• Skin (drug effects, metabolism)
• Surgical Flaps