The cardioprotective effect of salidroside against myocardial ischemia reperfusion injury in rats by inhibiting apoptosis and inflammation.

Abstract

The main purpose of this study was to investigate effect of salidroside (Sal) on myocardial ischemia reperfusion injury in rats and the underlying mechanism. Myocardial ischemia reperfusion injury (MI/RI) model was treated with 30 min of left anterior descending (LAD) occlusion followed by 24 h of reperfusion. The male Sprague-Dawley rats were randomly divided into 7 groups: (1) Sham; (2) Sham + diltiazem (Dit, 10 mg/kg); (3) Sham + Sal (40 mg/kg); (4) I/R; (5) I/R + diltiazem (Dit, 10 mg/kg); (6) I/R + Sal (20 mg/kg); (7) I/R + Sal (40 mg/kg). Sal could ameliorate myocardial ischemia reperfusion injury as evidenced by Histopathological examination and triphenyl tetrazolium chloride (TTC) staining. Moreover, terminal deoxynucleotidyl transferase dUTP nickend labeling (TUNEL) assay demonstrated that Sal suppressed myocardial apoptosis, which may be related to up-regulation of Bcl-2/Bax ratio and inhibition of caspase-3, caspase-9 activation. Pretreatment with Sal affected serum biochemical parameters and cardiac dysfunction compared with I/R group. Sal also attenuated the pro-inflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 in serum by inhibiting TLR4/NF-κB signaling pathway. Sal exerts strong favorable cardioprotective function on myocardial I/R injury which may relate to the down-regulation of the TLR4/NF-κB signaling pathway and the inhibition of cell apoptosis.

• Authors: Lingpeng Zhu, Tingting Wei, Jin Gao, Xiayun Chang, He He, Fen Luo, Rui Zhou, Chunhua Ma, Yu Liu, Tianhua Yan
• Journal: Apoptosis : an international journal on programmed cell death (Apoptosis) Vol. 20 Issue 11 Pg. 1433-43 (Nov 2015) ISSN: 1573-675X [Electronic] Netherlands
• PMID: 26385354 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glucosides
• Interleukin-1beta
• Interleukin-6
• Phenols
• Protective Agents
• Proto-Oncogene Proteins c-bcl-2
• Toll-Like Receptor 4
• Tumor Necrosis Factor-alpha
• bcl-2-Associated X Protein
• Caspase 3
• Caspase 9
• rhodioloside

Topics

• Animals
• Apoptosis (drug effects)
• Caspase 3 (genetics, immunology)
• Caspase 9 (genetics, immunology)
• Glucosides (administration & dosage)
• Humans
• Interleukin-1beta (genetics, immunology)
• Interleukin-6 (genetics, immunology)
• Male
• Myocardial Reperfusion Injury (drug therapy, genetics, immunology, physiopathology)
• Phenols (administration & dosage)
• Protective Agents (administration & dosage)
• Proto-Oncogene Proteins c-bcl-2 (genetics, immunology)
• Rats
• Rats, Sprague-Dawley
• Toll-Like Receptor 4 (genetics, immunology)
• Tumor Necrosis Factor-alpha (genetics, immunology)
• bcl-2-Associated X Protein (genetics, immunology)