Abstract | BACKGROUND: The blood pressure lowering effect of sesamin has been demonstrated to be associated with the increase in vascular nitric oxide (NO) biological activity by our previous studies and others. The present study was designed to explore the underlying mechanisms involved in the effect of sesamin on aortic NO bioactivity in spontaneously hypertensive rats (SHRs). METHODS: RESULTS: In SHRs, sesamin treatment reduced SBP, improved vascular relaxation induced by acetylcholine and enhanced aortic NO bioactivity. Sesamin treatment enhanced NO biosynthesis in SHR aortas was due to upregulated P-eNOS and suppressed eNOS uncoupling, and the latter effect might be attributed to decreased nitrotyrosine and upregulated DHFR. Sesamin also reduced the NO oxidative inactivation and decreased the superoxide anion production through downregulation of p47( phox) and amelioration of eNOS uncoupling. In addition, sesamin treatment did not alter the levels of GPx and catalase activity but obviously reduced the compensatory elevated T-SOD activity and Cu/Zn-SOD protein expression. CONCLUSION: Chronic treatment with sesamin could reduce hypertension and improve endothelial dysfunction through enhancement of NO bioactivity in SHR aortas.
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Authors | Xiang Kong, Wei Li, Li-qun Guo, Jun-xiu Zhang, Xiang-pan Chen, Wei-yong Liu, Jie-ren Yang |
Journal | Therapeutic advances in cardiovascular disease
(Ther Adv Cardiovasc Dis)
Vol. 9
Issue 5
Pg. 314-24
(Oct 2015)
ISSN: 1753-9455 [Electronic] England |
PMID | 26037786
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © The Author(s), 2015. |
Chemical References |
- Antihypertensive Agents
- Antioxidants
- Dioxoles
- Lignans
- Nitric Oxide
- Nitric Oxide Synthase Type III
- Superoxide Dismutase
- sesamin
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Topics |
- Animals
- Antihypertensive Agents
(pharmacology)
- Antioxidants
(metabolism)
- Aorta
(drug effects, metabolism)
- Blood Pressure
(drug effects)
- Blotting, Western
- Dioxoles
(pharmacology)
- Down-Regulation
(drug effects)
- Electrophoresis, Polyacrylamide Gel
- Endothelium, Vascular
(drug effects, metabolism)
- Hypertension
(drug therapy, physiopathology)
- Lignans
(pharmacology)
- Male
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase Type III
(metabolism)
- Rats
- Rats, Inbred SHR
- Rats, Inbred WKY
- Superoxide Dismutase
(metabolism)
- Up-Regulation
(drug effects)
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