Serum and synovial fluid levels of tumor necrosis factor-like ligand 1A and decoy receptor 3 in rheumatoid arthritis.

Abstract

OBJECTIVE:

To measure the levels of Tumor necrosis factor (TNF)-like ligand 1A (TL1A) and decoy receptor 3 (DcR3) in serum and synovial fluid (SF) of patients with rheumatoid arthritis (RA). To evaluate the effect of recombinant human (rh) TL1A on interleukin (IL)-17 production and IL-17mRNA expression.

METHODS:

The serum and SF levels of TL1A and DcR3, and the production of IL-17 by rhTL1A-treated PBMC were measured by enzyme-linked immunosorbent assay (ELISA). The expression of IL-17 mRNA by rhTL1A-treated PBMC was measured by real-time reverse transcriptase polymerase chain reaction (RT-PCR). We also tested the change of TL1A and DcR3 level following TNF-α blockade therapy.

RESULTS:

Serum TL1A and DcR3 levels were higher in RA patients. This increase was more significant in RF and anti-CCP positive patients. TL1A and DcR3 levels were higher in SF samples than in paired sera. TL1A and DcR3 decreased after anti-TNF treatment. rhTL1A increased the production of IL-17 protein and the expression of IL-17mRNA.

CONCLUSION:

TL1A and DcR3 may be of pathogenic and potentially of therapeutic importance in RA patients.

• Authors: Zijuan Xiu, Hui Shen, Ye Tian, Liping Xia, Jing Lu
• Journal: Cytokine (Cytokine) Vol. 72 Issue 2 Pg. 185-9 (Apr 2015) ISSN: 1096-0023 [Electronic] England
• PMID: 25647275 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Interleukin-17
• Receptors, Tumor Necrosis Factor, Member 6b
• TNFRSF6B protein, human
• Tumor Necrosis Factor Ligand Superfamily Member 15
• Tumor Necrosis Factor-alpha
• Infliximab

Topics

• Adult
• Aged
• Anti-Inflammatory Agents, Non-Steroidal
• Arthritis, Rheumatoid (immunology)
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Infliximab (administration & dosage, therapeutic use)
• Interleukin-17 (genetics, immunology)
• Leukocytes, Mononuclear (immunology, metabolism)
• Male
• Middle Aged
• Real-Time Polymerase Chain Reaction
• Receptors, Tumor Necrosis Factor, Member 6b (blood, metabolism)
• Synovial Fluid (immunology)
• Tumor Necrosis Factor Ligand Superfamily Member 15 (blood, metabolism)
• Tumor Necrosis Factor-alpha (antagonists & inhibitors)