Perillaldehyde attenuates cerebral ischemia-reperfusion injury-triggered overexpression of inflammatory cytokines via modulating Akt/JNK pathway in the rat brain cortex.

Abstract

Perillaldehyde (PAH), one of the major oil components in Perilla frutescens, has anti-inflammatory effects. Few studies have examined the neuroprotective effect of PAH on stroke. So the aim of our study is to investigate the effect of PAH on ischemia-reperfusion-induced injury in the rat brain cortex. Middle cerebral artery occlusion (MCAO) model was selected to make cerebral ischemia-reperfusion injury. Rats were assigned randomly to groups of sham, MCAO, and two treatment groups by PAH at 36.0, 72.0mg/kg. Disease model was set up after intragastrically (i.g.) administering for 7 consecutive days. The neurological deficit, the cerebral infarct size, biochemical parameters and the relative mRNA and protein levels were examined. The results showed that the NO level, the iNOS activity, the neurological deficit scores, the cerebral infarct size and the expression of inflammatory cytokines including interleukin (IL)-1β, interleukin (IL)-6 and tumor necrosis factor (TNF)-α were significantly decreased by PAH treatment. PAH also increased the Phospho-Akt level and decrease the Phospho-JNK level by Western blot analysis. Meanwhile, the PAH groups exhibited a dramatically decrease of apoptosis-related mRNA expression such as Bax and caspase-3. Our findings shown that PAH attenuates cerebral ischemia/reperfusion injury in the rat brain cortex, and suggest its neuroprotective effect is relate to regulating the inflammatory response through Akt /JNK pathway. The activation of this signalling pathway eventually inhibits apoptotic cell death induced by cerebral ischemia-reperfusion.

• Authors: Lixing Xu, Yuebi Li, Qiang Fu, Shiping Ma
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 454 Issue 1 Pg. 65-70 (Nov 07 2014) ISSN: 1090-2104 [Electronic] United States
• PMID: 25445600 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Bax protein, rat
• Cytokines
• Inflammation Mediators
• Interleukin-1beta
• Interleukin-6
• Monoterpenes
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• bcl-2-Associated X Protein
• perillaldehyde
• Proto-Oncogene Proteins c-akt
• Casp3 protein, rat
• Caspase 3

Topics

• Animals
• Brain Injuries (drug therapy, metabolism, pathology)
• Brain Ischemia (drug therapy, metabolism, pathology)
• Caspase 3 (genetics)
• Cytokines (metabolism)
• Disease Models, Animal
• Inflammation Mediators (blood)
• Interleukin-1beta (blood)
• Interleukin-6 (blood)
• MAP Kinase Signaling System (drug effects)
• Male
• Monoterpenes (pharmacology)
• Proto-Oncogene Proteins c-akt (metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy, metabolism, pathology)
• Tumor Necrosis Factor-alpha (blood)
• bcl-2-Associated X Protein (genetics)